PF3D7_1233300 (PPR2)

Genome location: Pf3D7_12_v3:1,375,225..1,379,661(+)

Genome classification: Core

Function and Localization

Product Description: pentatricopeptide repeat domain-containing protein 2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005739	mitochondrion

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.10	0.95
Trophozoite	possibly expressed	0.72	0.61
Schizont	possibly expressed	0.14	0.91
Gametocyte	possibly expressed	0.50	0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1233300

Resistome Missense Mutations: H1162N

Resistome Compounds with Missense Mutations: MMV982556

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.138 | MFS: -2.893 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1448000)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I589

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_122551

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	63	31	78
disruptive	63	166	82	239
missense	34	115	76	202

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	107	62	101
disruptive	100	328	172	393
missense	51	249	146	248

PlasmoDB Total SNPs: 199

Non-coding: 30 | Synonymous: 84 | Nonsynonymous: 84 | Stop Codon: 1

Protein Information

Protein Length: 1413 | Molecular Weight (kDa): 169.562

UniProt ID(s): A0A144A108, Q8I589

PDB ID(s): None

Isoelectric Point: 7.9

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002885	Pentatricopeptide repeat
PFam	PF13041	Pentatricopeptide repeat
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21
35657410	Loss of PPR protein Ppr2 induces ferroptosis-like cell death inSchizosaccharomyces pombe	Liu Z, Ebrahim A, ..., Huang Y	10.1007/s00203-022-02970-2