PF3D7_1233700

Genome location: Pf3D7_12_v3:1,402,635..1,405,063(+)

Genome classification: Core

Function and Localization

Product Description: homocysteine S-methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.10 (Homocysteine S-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0008898	S-adenosylmethionine-homocysteine S-methyltransferase activity
Process	GO:0033528	S-methylmethionine cycle
Process	GO:0009086	methionine biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.98
Trophozoite	not expressed	0.12	0.92
Schizont	not expressed	0.21	0.86
Gametocyte	not expressed	0.08	0.95

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1233700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.144 | MFS: -2.908 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1448300)

Relative Growth Rate: 0.96 ± 0.38
Confidence: 3.32

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1448300)

Modification: Disrupted | RMgm-4307

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I585

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.10	homocysteine S-methyltransferase	EDTAL-methionineS-adenosyl-L-methionineS-adenosyl-L-homocysteineN-Hydroxymethyl-DL-methionine

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533033

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	24	14	23
disruptive	21	77	47	96
missense	21	71	46	88

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	43	23	31
disruptive	44	166	71	150
missense	33	142	64	112

PlasmoDB Total SNPs: 92

Non-coding: 55 | Synonymous: 14 | Nonsynonymous: 23 | Stop Codon: 0

Protein Information

Protein Length: 581 | Molecular Weight (kDa): 68.955

UniProt ID(s): Q8I585

PDB ID(s): None

Isoelectric Point: 9.09

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003726	Homocysteine-binding domain
InterPro	IPR036589	Homocysteine-binding domain superfamily
PFam	PF02574	Homocysteine-binding domain
Superfamily	SSF82282	Homocysteine-binding domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104