Genome location: Pf3D7_12_v3:1,407,068..1,411,277(+)
Genome classification: Core
Product Description: sentrin-specific protease 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.22.68 (Ulp1 peptidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0016929 | SUMO-specific protease activity |
| Process | GO:0016926 | protein desumoylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.24 | 0.88 |
| Trophozoite | possibly expressed | 0.65 | 0.63 |
| Schizont | not expressed | 0.35 | 0.78 |
| Gametocyte | not expressed | 0.52 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1233900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.955 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1448500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I583
"Best" AlphaFill ligand hit: AYE (allylamine, Local RMSD=0.23) with 7R2E (Global RMSD=4.69)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.68 | Ulp1 peptidase | Gu-HClVEA-260hSUMO-VSN-ethylmaleimideUbiquitin aldehyde3-[(4-[[2-[(2E)-4-[bis(naphthalen-1-ylmethyl)amino]-4-oxobut-2-enoyl]-2-(carboxymethyl)hydrazinyl]carbonyl]benzyl)carbamoyl]benzoic acid3-[(4-[[2-[4-[bis(naphthalen-1-ylmethyl)amino]-2,3-dichloro-4-oxobutanoyl]-2-(carboxymethyl)hydrazinyl]carbonyl]benzyl)carbamoyl]benzoic acid3-[(4-[[2-[4-[bis(naphthalen-1-ylmethyl)amino]-3-chloro-2-hydroxy-4-oxobutanoyl]-2-(carboxymethyl)hydrazinyl]carbonyl]benzyl)carbamoyl]benzoic acid3-[(4-[[2-([(2R,3R)-3-[benzyl(cyclohexa-1,3-dien-1-ylmethyl)carbamoyl]-3-chlorooxiran-2-yl]carbonyl)-2-(carboxymethyl)hydrazinyl]carbonyl]benzyl)carbamoyl]benzoic acid |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q96HI0 | Sentrin-specific protease 5 | OrthoDB | CHEMBL4796113CHEMBL4780306CHEMBL4800446CHEMBL4785280... | |
| Q9P0U3 | Sentrin-specific protease 1 | Homo sapiens | OrthoDB | VEA-260VEA-499MLS000573775MLS000058000... |
| Q9HC62 | Sentrin-specific protease 2 | Homo sapiens | OrthoDB | JCP-666VEA-260VEA-499VEA-500... |
| Q9GZR1 | Sentrin-specific protease 6 | Homo sapiens | OrthoDB | MLS000073450MLS000027210MLS000029968MLS000070601... |
| Q9BQF6 | Sentrin-specific protease 7 | Homo sapiens | OrthoDB | VEA-561SPI-01SPI-02NSC8676... |
Ortholog Group (OrthoMCL): OG6_101235
Most Similar Human Ortholog: Q9P0U3
TM-align score: 0.41 | RMSD: 4.74
Seq Identity: 0.27 | Length: 303 / 1026
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000079387 | SUMO specific peptidase 1 |
Protein Length: 1026 | Molecular Weight (kDa): 123.225
UniProt ID(s): Q8I583
PDB ID(s): None
Isoelectric Point: 7.83
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003653 | Ulp1 protease family, C-terminal catalytic domain |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF02902 | Ulp1 protease family, C-terminal catalytic domain |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 51 | 24 | 64 |
| disruptive | 41 | 166 | 77 | 172 |
| missense | 24 | 119 | 67 | 142 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 92 | 41 | 64 |
| disruptive | 95 | 311 | 130 | 328 |
| missense | 45 | 236 | 108 | 210 |
PlasmoDB Total SNPs: 252
Non-coding: 142 | Synonymous: 51 | Nonsynonymous: 59 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21700207 | Functional characterization of a SUMO deconjugating protease of Plasmodiumfalciparum using newly identified small molecule inhibitors. | Ponder EL, Albrow VE, ..., Bogyo M | 10.1016/j.chembiol.2011.04.010 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 37914145 | SENP1 knockdown-mediated CTCF SUMOylation enhanced its stability and alleviatedlipopolysaccharide-evoked inflammatory injury in human lung fibroblasts viaregulation of FOXA2 transcription | Kang L, Wang X, ..., Lei R | 10.1016/j.bbagen.2023.130500 |