PF3D7_1234300

Genome location: Pf3D7_12_v3:1,433,803..1,436,007(-)

Genome classification: Core

Function and Localization

Product Description: DNA polymerase epsilon subunit B, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.7 (DNA-directed DNA polymerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0008622	epsilon DNA polymerase complex
Process	GO:0006261	DNA-dependent DNA replication
Process	GO:0042276	error-prone translesion synthesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.05	0.97
Trophozoite	possibly expressed	0.41	0.75
Schizont	possibly expressed	0.07	0.95
Gametocyte	not expressed	0.57	0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1234300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.125 | MFS: -3.201 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1448900)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I579

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.7	DNA-directed DNA polymerase	KClEDTANH4+dATPNH4ClddTTPMK886UracilalliinsucroseethanolAllicin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102104

Most Similar Human Ortholog: P56282

TM-align score: 0.77 | RMSD: 3.06

Seq Identity: 0.21 | Length: 442 / 624

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000100479	DNA polymerase epsilon 2, accessory subunit

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	16	15	26
disruptive	3	13	9	41
missense	3	13	9	40

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	46	26	34
disruptive	9	42	29	94
missense	4	33	25	70

PlasmoDB Total SNPs: 74

Non-coding: 51 | Synonymous: 19 | Nonsynonymous: 3 | Stop Codon: 1

Protein Information

Protein Length: 624 | Molecular Weight (kDa): 73.63

UniProt ID(s): Q8I579

PDB ID(s): None

Isoelectric Point: 6.42

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007185	DNA polymerase alpha/delta/epsilon, subunit B
InterPro	IPR016266	DNA polymerase epsilon, subunit B
PFam	PF04042	DNA polymerase alpha/delta/epsilon, subunit B
Superfamily	N/A	N/A