Genome location: Pf3D7_12_v3:1,451,196..1,457,549(+)
Genome classification: Core
Product Description: splicing factor 3B subunit 3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005686 | U2 snRNP |
| Component | GO:0005634 | nucleus |
| Component | GO:0005681 | spliceosomal complex |
| Process | GO:0008380 | RNA splicing |
| Process | GO:0000375 | RNA splicing, via transesterification reactions |
| Process | GO:0000398 | mRNA splicing, via spliceosome |
| Process | GO:0000245 | spliceosomal complex assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.41 | 0.80 |
| Trophozoite | expressed | 0.67 | 0.62 |
| Schizont | expressed | 0.13 | 0.92 |
| Gametocyte | expressed | 0.58 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1234800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.608 | MFS: -2.515 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1449400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I574
"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=3.03) with 4JSP (Global RMSD=15.37)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q15393 | Splicing factor 3B subunit 3 | Homo sapiens | HOGENOM, OMA, OrthoDB | CID16202130CID16202131CID16202132Pladienolide analog 8... |
Ortholog Group (OrthoMCL): OG6_102496
Most Similar Human Ortholog: J3QL37
TM-align score: 0.96 | RMSD: 0.52
Seq Identity: 0.63 | Length: 57 / 1329
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000189091 | splicing factor 3b subunit 3 |
Protein Length: 1329 | Molecular Weight (kDa): 151.812
UniProt ID(s): Q8I574
PDB ID(s): None
Isoelectric Point: 5.69
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004871 | Cleavage/polyadenylation specificity factor, A subunit, C-terminal |
| InterPro | IPR036322 | WD40-repeat-containing domain superfamily |
| PFam | PF03178 | Cleavage/polyadenylation specificity factor, A subunit, C-terminal |
| PFam | PF10433 | |
| Superfamily | SSF50978 | WD40-repeat-containing domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 54 | 39 | 86 |
| disruptive | 3 | 49 | 32 | 96 |
| missense | 3 | 49 | 29 | 95 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 124 | 47 | 105 |
| disruptive | 9 | 110 | 70 | 173 |
| missense | 6 | 98 | 68 | 137 |
PlasmoDB Total SNPs: 258
Non-coding: 195 | Synonymous: 48 | Nonsynonymous: 15 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 34281234 | A Transcriptome-Wide Isoform Landscape of Melanocytic Nevi and Primary MelanomasIdentifies Gene Isoforms Associated with Malignancy | Hakobyan S, Loeffler-Wirth H, ..., Kunz M | 10.3390/ijms22137165 |
| 34140643 | LINC01348 suppresses hepatocellular carcinoma metastasis through inhibition of SF3B3-mediated EZH2 pre-mRNA splicing | Lin YH, Wu MH, ..., Lin KH | 10.1038/s41388-021-01905-3 |
| 35028595 | Rewards of divergence in sequences, 3-D structures and dynamics of yeast andhuman spliceosome SF3b complexes | Yazhini A, Sandhya S, Srinivasan N | 10.1016/j.crstbi.2021.05.003 |
| 36357564 | Novel long noncoding RNA LINC02820 augments TNF signaling pathway to remodelcytoskeleton and potentiate metastasis in esophageal squamous cell carcinoma | Wang J, Huang TJ, ..., Huang BJ | 10.1038/s41417-022-00554-2 |
| 35306020 | Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors | Teng Z, Hao L, ..., Cheng Y | 10.1016/j.ijbiomac.2022.03.070 |
| 36572150 | Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection bytargeting GCH1 | Chen T, Yang H, ..., Peng X | 10.1016/j.virs.2022.12.005 |