About Gene List

PF3D7_1235300 (NOT4)

Genome location: Pf3D7_12_v3:1,469,044..1,476,429(+)

Genome classification: Core

Function and Localization

Product Description: CCR4-NOT transcription complex subunit 4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0030014 CCR4-NOT complex
Component GO:0005634 nucleus
Function GO:0003729 mRNA binding
Function GO:0004842 ubiquitin-protein transferase activity
Process GO:0016567 protein ubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 1.72 0.33
Trophozoite expressed 1.67 0.27
Schizont possibly expressed 1.14 0.45
Gametocyte possibly expressed 1.56 0.30

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1235300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.364 | MFS: -2.642 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I569

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102042

Most Similar Human Ortholog: O95628

TM-align score: 0.38 | RMSD: 6.46

Seq Identity: 0.26 | Length: 290 / 1662

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000080802 CCR4-NOT transcription complex subunit 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 30 62 46 91
disruptive 66 215 134 298
missense 36 140 100 215

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 42 146 70 95
disruptive 146 525 255 600
missense 65 335 181 316

PlasmoDB Total SNPs: 417

Non-coding: 189 | Synonymous: 129 | Nonsynonymous: 98 | Stop Codon: 1

Protein Information

Protein Length: 1662 | Molecular Weight (kDa): 194.674

UniProt ID(s): Q8I569

PDB ID(s): None

Isoelectric Point: 7.82

Protein Domain Annotations:

Source Family ID Description
InterPro IPR003954 RNA recognition motif domain, eukaryote
InterPro IPR034261 CNOT4, RNA recognition motif
InterPro IPR035979 RNA-binding domain superfamily
InterPro IPR039515 NOT4, modified RING finger, HC subclass (C4C4-type)
InterPro IPR039780 CCR4-NOT transcription complex subunit 4
PFam PF14570 N/A
Superfamily SSF54928 RNA-binding domain superfamily
Superfamily SSF57850

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
27381095 The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. Bunnik EM, Batugedara G, ..., Le Roch KG 10.1186/s13059-016-1014-0
34628507 Corrigendum to: The C-terminal region of yeast ubiquitin-protein ligase Not4mediates its cellular localization and stress response Mazzoni C 10.1093/femsle/fnab122
33184379 Ribosomal protein S7 ubiquitination during ER stress in yeast is associated withselective mRNA translation and stress outcome Matsuki Y, Matsuo Y, ..., Inada T 10.1038/s41598-020-76239-3
34338747 The C-terminal region of yeast ubiquitin-protein ligase Not4 mediates itscellular localization and stress response Palermo V, Stirpe M, ..., Mazzoni C 10.1093/femsle/fnab097