PF3D7_1235400

Genome location: Pf3D7_12_v3:1,476,470..1,480,044(-)

Genome classification: Core

Function and Localization

Product Description: tetQ family GTPase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0003924	GTPase activity
Process	GO:0032543	mitochondrial translation
Process	GO:0032790	ribosome disassembly
Process	GO:0006414	translational elongation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.09	0.95
Trophozoite	possibly expressed	0.31	0.81
Schizont	possibly expressed	0.08	0.95
Gametocyte	possibly expressed	0.30	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1235400

Resistome Missense Mutations: T106P

Resistome Compounds with Missense Mutations: Desoxyepothilone B

Resistome # Samples with Disruptive Mutations: 8 (8 missense, 8 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.214 | MFS: -2.711 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1450000)

Relative Growth Rate: 0.91 ± 0.10
Confidence: 5.98

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1450000)

Modification: Disrupted | RMgm-3998

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I568

"Best" AlphaFill ligand hit: OGA (n-oxalylglycine, Local RMSD=0.02) with 4IW3 (Global RMSD=7.43)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532693

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	46	15	57
disruptive	37	138	64	200
missense	21	104	50	161

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	14	92	41	65
disruptive	65	292	146	279
missense	37	205	104	177

PlasmoDB Total SNPs: 202

Non-coding: 6 | Synonymous: 67 | Nonsynonymous: 129 | Stop Codon: 0

Protein Information

Protein Length: 1161 | Molecular Weight (kDa): 136.381

UniProt ID(s): C5J098, C5J0A4, C5J0A9, C5J0B0, C5J0C6, C5J0D2, C5J0D3, C5J0E5, C5J0F3, C5J0F5, Q8I568

PDB ID(s): None

Isoelectric Point: 7.93

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000640	Elongation factor EFG, domain V-like
InterPro	IPR000795	Transcription factor, GTP-binding domain
InterPro	IPR005225	Small GTP-binding protein domain
InterPro	IPR009000	Translation protein, beta-barrel domain superfamily
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR031157	Tr-type G domain, conserved site
InterPro	IPR035647	EF-G domain III/V-like
InterPro	IPR041095	Elongation Factor G, domain II
PFam	PF00009	Transcription factor, GTP-binding domain
PFam	PF00679	Elongation factor EFG, domain V-like
PFam	PF14492	Elongation Factor G, domain II
Superfamily	SSF50447	Translation protein, beta-barrel domain superfamily
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase
Superfamily	SSF54980	EF-G domain III/V-like

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
19929377	Susceptibility of Plasmodium falciparum isolates to doxycycline is associatedwith pftetQ sequence polymorphisms and pftetQ and pfmdt copy numbers.	Briolant S, Wurtz N, ..., Pradines B	10.1086/648594
26206143	Absence of correlation between ex vivo susceptibility to doxycycline andpfteQ-pfmdt gene polymorphism in French Guiana.	Mura M, Briolant S, ..., Legrand E	10.1186/s12936-015-0788-y