Genome location: Pf3D7_12_v3:1,480,516..1,482,959(+)
Genome classification: Core
Product Description: N6-adenosine-methyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.62 (mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0036396 | RNA N6-methyladenosine methyltransferase complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003729 | mRNA binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0080009 | mRNA methylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.22 | 0.89 |
| Trophozoite | expressed | 0.41 | 0.75 |
| Schizont | expressed | 0.14 | 0.91 |
| Gametocyte | expressed | 0.36 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1235500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.498 | MFS: -2.51 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1450100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I567
"Best" AlphaFill ligand hit: ADN (adenosine, Local RMSD=0.53) with 6TTP (Global RMSD=2.86)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.62 | mRNA (2'-O-methyladenosine-N6-)-methyltransferase | N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9HCE5 | N6-adenosine-methyltransferase catalytic/non-catalytic subunit | OrthoDB, OrthoMCL BLAST | US11725010, Example 1US11725010, Example 47US11725010, Example 3US11725010, Example 4... | |
| Q86U44 | N6-adenosine-methyltransferase catalytic subunit | OrthoDB | CHEMBL4854665CHEMBL4850503CHEMBL4872977CHEMBL4856985... |
Ortholog Group (OrthoMCL): OG6_104881
Most Similar Human Ortholog: Q9HCE5
TM-align score: 0.65 | RMSD: 2.92
Seq Identity: 0.46 | Length: 315 / 646
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000145388 | methyltransferase like 14 |
Protein Length: 646 | Molecular Weight (kDa): 75.052
UniProt ID(s): Q8I567
PDB ID(s): None
Isoelectric Point: 7.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002052 | DNA methylase, N-6 adenine-specific, conserved site |
| InterPro | IPR007757 | MT-A70-like |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF05063 | MT-A70-like |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 38 | 23 | 41 |
| disruptive | 11 | 46 | 24 | 72 |
| missense | 6 | 37 | 22 | 62 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 78 | 24 | 32 |
| disruptive | 21 | 98 | 55 | 109 |
| missense | 10 | 81 | 45 | 82 |
PlasmoDB Total SNPs: 71
Non-coding: 32 | Synonymous: 29 | Nonsynonymous: 8 | Stop Codon: 2
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 31384004 | Transcriptome-wide dynamics of extensive m(6)A mRNA methylation during Plasmodiumfalciparum blood-stage development. | Baumgarten S, Bryant JM, ..., Scherf A | 10.1038/s41564-019-0521-7 |