PF3D7_1235700

Genome location: Pf3D7_12_v3:1,486,145..1,489,166(+)

Genome classification: Core

Function and Localization

Product Description: ATP synthase subunit beta, mitochondrial

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.3.14 (Transferred entry: 7.1.2.2);7.1.2.2 (H(+)-transporting two-sector ATPase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005753	mitochondrial proton-transporting ATP synthase complex
Component	GO:0005739	mitochondrion
Component	GO:0005634	nucleus
Component	GO:0045261	proton-transporting ATP synthase complex, catalytic core F(1)
Process	GO:0042776	mitochondrial ATP synthesis coupled proton transport
Process	GO:1902600	proton transmembrane transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.03	0.98
Trophozoite	expressed	0.36	0.77
Schizont	expressed	0.07	0.95
Gametocyte	expressed	0.33	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1235700

Resistome Missense Mutations: V325I, R331S, I332M, D345E, L346I, A348E, A348P, L435I

Resistome Compounds with Missense Mutations: AN13762, MMV407834

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.622 | MFS: -2.512 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1450300)

Relative Growth Rate: 0.78 ± 0.18
Confidence: 4.83

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1450300)

Modification: Disrupted | RMgm-4000

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I0V2

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.01) with 1W0J (Global RMSD=1.77)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.3.14	H+-transporting two-sector ATPase	No BRENDA inhibitors
7.1.2.2	H+-transporting two-sector ATPase	BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P00830	ATP synthase subunit beta, mitochondrial	Saccharomyces cerevisiae	HOGENOM, OMA, OrthoDB, OrthoMCL	Apoptolidin ACHEMBL1077465CHEMBL1077466CHEMBL1077467...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100608

Most Similar Human Ortholog: P06576

TM-align score: 0.89 | RMSD: 1.07

Seq Identity: 0.76 | Length: 476 / 535

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000110955	ATP synthase F1 subunit beta

Protein Information

Protein Length: 535 | Molecular Weight (kDa): 58.394

UniProt ID(s): Q8I0V2

PDB ID(s): None

Isoelectric Point: 6.36

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000194	ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain
InterPro	IPR003593	AAA+ ATPase domain
InterPro	IPR004100	ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain
InterPro	IPR005722	ATP synthase, F1 complex, beta subunit
InterPro	IPR020003	ATPase, alpha/beta subunit, nucleotide-binding domain, active site
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR036121	ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily
PFam	PF00006	ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain
PFam	PF02874	ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain
Superfamily	SSF47917	ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily
Superfamily	SSF50615	P-loop containing nucleoside triphosphate hydrolase
Superfamily	SSF52540

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	25	12	23
disruptive	0	12	6	18
missense	0	11	5	18

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	54	14	23
disruptive	3	22	16	48
missense	1	18	15	40

PlasmoDB Total SNPs: 91

Non-coding: 81 | Synonymous: 8 | Nonsynonymous: 2 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
21984828	ATP synthase complex of Plasmodium falciparum: dimeric assembly in mitochondrialmembranes and resistance to genetic disruption.	Nina PB, Morrisey JM, ..., Vaidya AB	10.1074/jbc.M111.290973
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21