PF3D7_1236000

Genome location: Pf3D7_12_v3:1,502,375..1,504,236(+)

Genome classification: Core

Function and Localization

Product Description: vesicle transport v-SNARE protein VTI1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0012507	ER to Golgi transport vesicle membrane
Component	GO:0005794	Golgi apparatus
Component	GO:0031201	SNARE complex
Component	GO:0005789	endoplasmic reticulum membrane
Component	GO:0031902	late endosome membrane
Function	GO:0005484	SNAP receptor activity
Function	GO:0000149	SNARE binding
Process	GO:0006896	Golgi to vacuole transport
Process	GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport
Process	GO:0006891	intra-Golgi vesicle-mediated transport
Process	GO:0006623	protein targeting to vacuole
Process	GO:0042147	retrograde transport, endosome to Golgi
Process	GO:0048280	vesicle fusion with Golgi apparatus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.12	0.94
Trophozoite	expressed	0.63	0.64
Schizont	expressed	0.27	0.83
Gametocyte	expressed	0.88	0.52

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1236000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.132 | MFS: -2.891 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1450600)

Relative Growth Rate: 0.05 ± 0.07
Confidence: 6.78

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I563

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101479

Most Similar Human Ortholog: Q96AJ9

TM-align score: 0.47 | RMSD: 3.25

Seq Identity: 0.09 | Length: 121 / 410

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000151532	vesicle transport through interaction with t-SNAREs 1A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	15	13	27
disruptive	11	33	25	60
missense	11	32	24	56

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	48	17	22
disruptive	20	89	65	123
missense	17	78	54	86

PlasmoDB Total SNPs: 88

Non-coding: 68 | Synonymous: 9 | Nonsynonymous: 11 | Stop Codon: 0

Protein Information

Protein Length: 410 | Molecular Weight (kDa): 49.146

UniProt ID(s): Q8I563

PDB ID(s): None

Isoelectric Point: 5.76

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007705	Vesicle transport v-SNARE, N-terminal
PFam	PF05008	Vesicle transport v-SNARE, N-terminal
Superfamily	N/A	N/A