PF3D7_1236600

Genome location: Pf3D7_12_v3:1,527,455..1,528,034(-)

Genome classification: Core

Function and Localization

Product Description: p25-alpha family protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0032273	positive regulation of protein polymerization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.00	1.00
Trophozoite	expressed	0.00	1.00
Schizont	expressed	0.01	1.00
Gametocyte	expressed	0.34	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1236600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.12 | MFS: -2.715 | #Insertions: 0

PlasmoGEM Phenotype: Fast (Pb ortholog: PBANKA_1451200)

Relative Growth Rate: 1.07 ± 0.06
Confidence: 7.17

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_1453700)

Modification: Disrupted | RMgm-5261

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I557

"Best" AlphaFill ligand hit: MA5 (cyclohexylethyl-beta-d-maltoside, Local RMSD=0.59) with 2AZN (Global RMSD=14.72)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O94811	Tubulin polymerization-promoting protein	Homo sapiens	OrthoDB, OrthoMCL BLAST	Boronic acid, trans-6Boronic acid, trans-6combretastatin A-4, (CSA4)Boronic acid, trans-6...
Q27957	Tubulin polymerization-promoting protein		OrthoMCL BLAST	7alphaH-colchicineCHEMBL31779CHEMBL31663CHEMBL28250

Orthology Information

Ortholog Group (OrthoMCL): OG6_102553

Most Similar Human Ortholog: H0Y8L2

TM-align score: 0.68 | RMSD: 1.49

Seq Identity: 0.31 | Length: 90 / 152

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000159713	tubulin polymerization promoting protein family member 3
ENSG00000171368	tubulin polymerization promoting protein
ENSG00000179636	tubulin polymerization promoting protein family member 2

Protein Information

Protein Length: 152 | Molecular Weight (kDa): 17.398

UniProt ID(s): Q8I557

PDB ID(s): None

Isoelectric Point: 9.49

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR008907	P25-alpha
InterPro	IPR011992	EF-hand domain pair
PFam	PF05517	P25-alpha
Superfamily	SSF47473	EF-hand domain pair

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	9	4	10
disruptive	2	10	7	13
missense	2	10	7	10

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	18	5	14
disruptive	4	27	11	31
missense	3	25	10	18

PlasmoDB Total SNPs: 14

Non-coding: 8 | Synonymous: 2 | Nonsynonymous: 4 | Stop Codon: 0