Product Description: S-adenosyl-methyltransferase, putative
SignalP Peptide: MLTFWTCLIFVLFLDIYDC
# Transmembrane Domains: 0
EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.12 (Methionine S-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0071424 | rRNA (cytosine-N4-)-methyltransferase activity |
| Process | GO:0070475 | rRNA base methylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.09 | 0.95 |
| Trophozoite | expressed | 0.46 | 0.73 |
| Schizont | expressed | 0.24 | 0.83 |
| Gametocyte | possibly expressed | 0.14 | 0.92 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1236700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.152 | MFS: -3.176 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I556
"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.18) with 1N2X (Global RMSD=2.56)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.- | Ex: homocysteine S-methyltransferase | EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate... |
| 2.1.1.12 | methionine S-methyltransferase | NEMD-MetGly-MetL-ethionineL-methioninolN-formyl-L-MetL-MethioninamideL-Met ethyl esterL-Met methyl esterseleno-L-methionineL-Met p-nitroanilideS-methyl-L-methionine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101712
Most Similar Human Ortholog: A6NJ78
TM-align score: 0.73 | RMSD: 2.73
Seq Identity: 0.29 | Length: 322 / 508
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000169519 | methyltransferase like 15 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 20 | 20 | 35 |
| disruptive | 10 | 54 | 45 | 71 |
| missense | 10 | 53 | 44 | 63 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 48 | 21 | 36 |
| disruptive | 29 | 141 | 67 | 111 |
| missense | 24 | 116 | 60 | 93 |
PlasmoDB Total SNPs: 106
Non-coding: 70 | Synonymous: 27 | Nonsynonymous: 8 | Stop Codon: 1
Protein Length: 508 | Molecular Weight (kDa): 59.964
UniProt ID(s): Q8I556
PDB ID(s): None
Isoelectric Point: 9.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002903 | Ribosomal RNA small subunit methyltransferase H |
| InterPro | IPR023397 | S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF01795 | Ribosomal RNA small subunit methyltransferase H |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| Superfamily | SSF81799 | S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |