About Gene List

PF3D7_1236800 (ICMT)

Genome location: Pf3D7_12_v3:1,531,958..1,534,077(+)

Genome classification: Core

Function and Localization

Product Description: protein-S-isoprenylcysteine O-methyltransferase

SignalP Peptide: N/A

# Transmembrane Domains: 6

EC Numbers: 2.1.1.100 (Protein-S-isoprenylcysteine O-methyltransferase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005783 endoplasmic reticulum
Function GO:0004671 protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity
Process GO:0006481 C-terminal protein methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.04 0.98
Trophozoite not expressed 0.42 0.75
Schizont possibly expressed 0.38 0.76
Gametocyte possibly expressed 0.55 0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1236800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.418 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I555

"Best" AlphaFill ligand hit: D10 (decane, Local RMSD=0.01) with 5V7P (Global RMSD=12.73)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.1.1.100 protein-S-isoprenylcysteine O-methyltransferase CTABTween 80FTI-2628adenosinesinefunginNonidet P40cysmethynil(S)-glabrolTriton X-100deoxycholateaplysamine 6Phenylglyoxal...

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P32584 Protein-S-isoprenylcysteine O-methyltransferase Saccharomyces cerevisiae OrthoDB, OrthoMCL CHEMBL198274((2E,6E)-3,7,11-Trimethyl-dodeca-2,6,10-trienylsulfanyl)-acetic acid anion
Q9WVM4 Protein-S-isoprenylcysteine O-methyltransferase Rattus norvegicus OrthoDB CHEMBL23289Farnesylthiosalicyclic acidCHEMBL23751
O60725 Protein-S-isoprenylcysteine O-methyltransferase Homo sapiens OrthoDB CHEMBL396652CHEMBL3822531CHEMBL3822531CHEMBL2035530...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101638

Most Similar Human Ortholog: O60725

TM-align score: 0.79 | RMSD: 2.93

Seq Identity: 0.22 | Length: 254 / 509

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000116237 isoprenylcysteine carboxyl methyltransferase

Protein Information

Protein Length: 509 | Molecular Weight (kDa): 62.431

UniProt ID(s): Q8I555

PDB ID(s): None

Isoelectric Point: 9.83

Protein Domain Annotations:

Source Family ID Description
InterPro IPR007269 Isoprenylcysteine carboxyl methyltransferase
PFam PF04140 Isoprenylcysteine carboxyl methyltransferase
Superfamily N/A N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 5 20 15 26
disruptive 4 43 25 57
missense 4 42 23 54

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 8 42 19 33
disruptive 7 83 59 89
missense 7 69 51 72

PlasmoDB Total SNPs: 0

Non-coding: 0 | Synonymous: 0 | Nonsynonymous: 0 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
37667642 Identification of the ATPase alpha subunit of Trypanosoma cruzi as a potentialbiomarker for the diagnosis of Chagas disease Morales-Velasquez M, Baron-Vera JP, ..., Ospina-Villa JD 10.1080/1354750X.2023.2255756
38191574 Sustained rhoptry docking and discharge requires Toxoplasma gondii intraconoidalmicrotubule-associated proteins Dos Santos Pacheco N, Tell I Puig A, ..., Soldati-Favre D 10.1038/s41467-023-44631-y