Genome location: Pf3D7_12_v3:1,537,454..1,540,646(+)
Genome classification: Core
Product Description: SUMO-activating enzyme subunit 2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.2.1.45 (E1 ubiquitin-activating enzyme)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031510 | SUMO activating enzyme complex |
| Component | GO:0005737 | cytoplasm |
| Function | GO:0019948 | SUMO activating enzyme activity |
| Function | GO:0004839 | ubiquitin activating enzyme activity |
| Process | GO:0032446 | protein modification by small protein conjugation |
| Process | GO:0016925 | protein sumoylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.11 | 0.95 |
| Trophozoite | expressed | 0.51 | 0.70 |
| Schizont | expressed | 0.14 | 0.91 |
| Gametocyte | not expressed | 0.22 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1237000
Resistome Missense Mutations: Q62L, H184D, A391E
Resistome Compounds with Missense Mutations: AN13762, AN13956
Resistome # Samples with Disruptive Mutations: 4 (4 missense, 4 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.159 | MFS: -2.872 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1451600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I553
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.18) with 1Y8Q (Global RMSD=3.25)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.45 | E1 ubiquitin-activating enzyme | LMO2PYR-41AtMUB3largazoletrichostatin Aginsenoside Reginsenoside Rg1[ubiquitin carrier protein Ubc4]-L-cysteine5'-[[(L-cysteinylglycylglycyl)sulfamoyl]amino]-5'-deoxyadenosine1-(3-chloro-4-fluorophenyl)-4-[(5-nitro-2-furyl)methylene]-3,5-pyrazolidinedione4[4-(5-nitro-furan-2-ylmethylene)-3,5-dioxo-pyrazolidin-1-yl]-benzoic acid ethyl ester(5R,8S)-11-ethenyl-5-methyl-8-(propan-2-yl)-10-oxa-3,17-dithia-7,14,19,20-tetraazatricyclo[14.2.1.1~2,5~]icosa-1(18),2(20),16(19)-triene-6,9,13-trione... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9UBT2 | SUMO-activating enzyme subunit 2 | OMA, OrthoDB | US9796725, Compound I-001US9796725, Compound I-098US9796725, Compound I-003US9796725, Compound I-004... | |
| A0AVT1 | Ubiquitin-like modifier-activating enzyme 6 | Homo sapiens | OrthoDB | US9593121, 1-01US9593121, I-02US9593121, I-03US9593121, I-04... |
| P41226 | Ubiquitin-like modifier-activating enzyme 7 | Homo sapiens | OrthoDB | CHEMBL2017005 |
| P22314 | Ubiquitin-like modifier-activating enzyme 1 | Homo sapiens | OrthoDB | Compound ICompound ICompound IUS9796725, Compound I-001... |
| Q8TBC4 | NEDD8-activating enzyme E1 catalytic subunit | OrthoDB | MITOXANTRONEPevonedistatCHEMBL4878088CHEMBL4865086... |
Ortholog Group (OrthoMCL): OG6_101769
Most Similar Human Ortholog: K7EPL2
TM-align score: 0.79 | RMSD: 2.62
Seq Identity: 0.34 | Length: 228 / 686
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000126261 | ubiquitin like modifier activating enzyme 2 |
Protein Length: 686 | Molecular Weight (kDa): 81.12
UniProt ID(s): Q8I553
PDB ID(s): None
Isoelectric Point: 6.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000594 | THIF-type NAD/FAD binding fold |
| InterPro | IPR028077 | Ubiquitin/SUMO-activating enzyme ubiquitin-like domain |
| InterPro | IPR030661 | SUMO-activating enzyme subunit Uba2 |
| InterPro | IPR035985 | Ubiquitin-activating enzyme |
| PFam | PF00899 | THIF-type NAD/FAD binding fold |
| PFam | PF14732 | Ubiquitin/SUMO-activating enzyme ubiquitin-like domain |
| Superfamily | SSF69572 | Ubiquitin-activating enzyme |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 22 | 15 | 47 |
| disruptive | 20 | 75 | 48 | 112 |
| missense | 10 | 63 | 41 | 98 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 56 | 28 | 57 |
| disruptive | 39 | 176 | 72 | 169 |
| missense | 15 | 143 | 59 | 110 |
PlasmoDB Total SNPs: 151
Non-coding: 112 | Synonymous: 18 | Nonsynonymous: 21 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23943616 | Identification of biochemically distinct properties of the smallubiquitin-related modifier (SUMO) conjugation pathway in Plasmodium falciparum. | Reiter K, Mukhopadhyay D, ..., Matunis MJ | 10.1074/jbc.M113.498410 |