PF3D7_1237400

Genome location: Pf3D7_12_v3:1,558,660..1,560,695(+)

Genome classification: Core

Function and Localization

Product Description: hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.13	0.93
Trophozoite	not expressed	0.36	0.78
Schizont	not expressed	0.20	0.86
Gametocyte	not expressed	0.24	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1237400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.06 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I549

"Best" AlphaFill ligand hit: 5GP (guanosine-5'-monophosphate, Local RMSD=0.31) with 3MD7 (Global RMSD=2.34)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103649

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	26	16	23
disruptive	17	50	23	43
missense	17	50	23	39

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	52	14	37
disruptive	33	87	47	116
missense	29	78	37	86

PlasmoDB Total SNPs: 108

Non-coding: 76 | Synonymous: 11 | Nonsynonymous: 21 | Stop Codon: 0

Protein Information

Protein Length: 438 | Molecular Weight (kDa): 51.296

UniProt ID(s): Q8I549

PDB ID(s): None

Isoelectric Point: 6.98

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001279	Metallo-beta-lactamase
InterPro	IPR036866	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like
PFam	PF12706	Metallo-beta-lactamase
Superfamily	SSF56281	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like