Genome location: Pf3D7_12_v3:1,588,614..1,592,466(+)
Genome classification: Core
Product Description: pre-mRNA-splicing factor CWC22, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005684 | U2-type spliceosomal complex |
| Component | GO:0071013 | catalytic step 2 spliceosome |
| Function | GO:0003723 | RNA binding |
| Process | GO:0000398 | mRNA splicing, via spliceosome |
| Process | GO:0051726 | regulation of cell cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.14 | 0.51 |
| Trophozoite | expressed | 1.07 | 0.46 |
| Schizont | expressed | 0.24 | 0.86 |
| Gametocyte | possibly expressed | 0.58 | 0.69 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1238300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.132 | MFS: -2.84 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1452800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I540
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101743
Most Similar Human Ortholog: Q9HCG8
TM-align score: 0.43 | RMSD: 6.53
Seq Identity: 0.28 | Length: 489 / 967
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000163510 | CWC22 spliceosome associated protein homolog |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 35 | 16 | 56 |
| disruptive | 70 | 146 | 71 | 154 |
| missense | 36 | 100 | 63 | 122 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 72 | 43 | 42 |
| disruptive | 105 | 271 | 92 | 189 |
| missense | 52 | 205 | 78 | 126 |
PlasmoDB Total SNPs: 240
Non-coding: 50 | Synonymous: 94 | Nonsynonymous: 96 | Stop Codon: 0
Protein Length: 967 | Molecular Weight (kDa): 112.855
UniProt ID(s): Q8I540
PDB ID(s): None
Isoelectric Point: 9.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003890 | MIF4G-like, type 3 |
| InterPro | IPR003891 | Initiation factor eIF-4 gamma, MA3 |
| InterPro | IPR016024 | Armadillo-type fold |
| PFam | PF02847 | Initiation factor eIF-4 gamma, MA3 |
| Superfamily | SSF48371 | Armadillo-type fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 30696855 | Exon junction complex components Y14 and Mago still play a role in budding yeast | Boisrame A, Devillers H, ..., Neuveglise C | 10.1038/s41598-018-36785-3 |
| 28919079 | Structure of an Intron Lariat Spliceosome from Saccharomyces cerevisiae | Wan R, Yan C, ..., Shi Y | 10.1016/j.cell.2017.08.029 |