Genome location: Pf3D7_12_v3:1,600,643..1,603,876(-)
Genome classification: Core
Product Description: sphingomyelin phosphodiesterase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.4.12 (Sphingomyelin phosphodiesterase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004518 | nuclease activity |
| Function | GO:0004620 | phospholipase activity |
| Function | GO:0004767 | sphingomyelin phosphodiesterase activity |
| Process | GO:0006684 | sphingomyelin metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.07 | 0.96 |
| Trophozoite | expressed | 0.03 | 0.98 |
| Schizont | expressed | 0.11 | 0.92 |
| Gametocyte | expressed | 0.02 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1238600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.894 | #Insertions: 2
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I537
"Best" AlphaFill ligand hit: DGA (diacyl glycerol, Local RMSD=0.22) with 3I5V (Global RMSD=2.64)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.4.12 | sphingomyelin phosphodiesterase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P11889 | Sphingomyelinase C | OrthoMCL BLAST | RY221B-aSMY-471SMY-540SMY-610 |
Protein Length: 393 | Molecular Weight (kDa): 46.013
PDB ID(s): None
Isoelectric Point: 9.27
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR036691 | Endonuclease/exonuclease/phosphatase superfamily |
| PFam | N/A | N/A |
| Superfamily | SSF56219 | Endonuclease/exonuclease/phosphatase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 23 | 15 | 23 |
| disruptive | 0 | 8 | 2 | 29 |
| missense | 0 | 8 | 2 | 26 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 47 | 19 | 30 |
| disruptive | 2 | 28 | 34 | 108 |
| missense | 1 | 23 | 30 | 78 |
PlasmoDB Total SNPs: 91
Non-coding: 86 | Synonymous: 5 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11781362 | Plasmodium falciparum phospholipase C hydrolyzing sphingomyelin andlysocholinephospholipids is a possible target for malaria chemotherapy. | Hanada K, Palacpac NM, ..., Mitamura T | 10.1084/jem.20010724 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 29914511 | Identification and characterization of DNA endonucleases in Plasmodium falciparum3D7 clone. | Jiang N, Tu Z, ..., Chen Q | 10.1186/s12936-018-2388-0 |