Genome location: Pf3D7_12_v3:1,608,501..1,614,434(+)
Genome classification: Core
Product Description: acyl-CoA synthetase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0016020 | membrane |
| Component | GO:0005634 | nucleus |
| Function | GO:0004467 | long-chain fatty acid-CoA ligase activity |
| Process | GO:0006631 | fatty acid metabolic process |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.86 | 0.28 |
| Trophozoite | expressed | 1.31 | 0.37 |
| Schizont | expressed | 0.93 | 0.49 |
| Gametocyte | expressed | 0.61 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1238800
Resistome Missense Mutations: S74L, D269G, F387V, D648Y, E668K, T767I
Resistome Compounds with Missense Mutations: MMV019719, MMV019721, MMV665924
Resistome # Samples with Disruptive Mutations: 12 (11 missense, 10 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.131 | MFS: -2.956 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1453300)
Modification: Disrupted | RMgm-4009
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I535
"Best" AlphaFill ligand hit: 3UK (5'-o-[(s)-[(2-aminobenzoyl)oxy](hydroxy)phosphoryl]adenosine, Local RMSD=0.62) with 4WV3 (Global RMSD=6.02)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.3 | long-chain-fatty-acid-CoA ligase | ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100152
Most Similar Human Ortholog: B7Z3Z9
TM-align score: 0.92 | RMSD: 2.32
Seq Identity: 0.31 | Length: 520 / 792
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000151726 | acyl-CoA synthetase long chain family member 1 |
| ENSG00000164398 | acyl-CoA synthetase long chain family member 6 |
| ENSG00000197142 | acyl-CoA synthetase long chain family member 5 |
| ENSG00000281938 | novel transcript |
Protein Length: 792 | Molecular Weight (kDa): 92.092
PDB ID(s): None
Isoelectric Point: 9.03
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000873 | AMP-dependent synthetase/ligase |
| InterPro | IPR020845 | AMP-binding, conserved site |
| PFam | PF00501 | AMP-dependent synthetase/ligase |
| Superfamily | SSF56801 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 48 | 19 | 61 |
| disruptive | 21 | 68 | 37 | 113 |
| missense | 9 | 37 | 31 | 73 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 98 | 29 | 58 |
| disruptive | 38 | 192 | 79 | 226 |
| missense | 12 | 112 | 59 | 123 |
PlasmoDB Total SNPs: 154
Non-coding: 85 | Synonymous: 53 | Nonsynonymous: 16 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 29326268 | Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics. | Cowell AN, Istvan ES, ..., Winzeler EA | 10.1126/science.aan4472 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |