Genome location: Pf3D7_12_v3:1,612,601..1,618,965(-)
Genome classification: Core
Product Description: protein kinase 2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.- (Phosphotransferases with an alcohol group as acceptor.);2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0016020 | membrane |
| Component | GO:0005634 | nucleus |
| Function | GO:0005509 | calcium ion binding |
| Function | GO:0009931 | calcium-dependent protein serine/threonine kinase activity |
| Function | GO:0005516 | calmodulin binding |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0018105 | peptidyl-serine phosphorylation |
| Process | GO:0046777 | protein autophosphorylation |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.32 | 0.89 |
| Trophozoite | expressed | 0.07 | 0.96 |
| Schizont | expressed | 0.25 | 0.89 |
| Gametocyte | expressed | 0.06 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1238900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -2.888 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I534
"Best" AlphaFill ligand hit: CPS (chaps, Local RMSD=0.07) with 4CZT (Global RMSD=5.56)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 1 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 1 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 430
Non-coding: 386 | Synonymous: 30 | Nonsynonymous: 14 | Stop Codon: 0
Protein Length: 509 | Molecular Weight (kDa): 58.915
UniProt ID(s): Q8I534
PDB ID(s): None
Isoelectric Point: 8.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 1378403 | Molecular cloning, stage-specific expression and cellular distribution of aputative protein kinase from Plasmodium falciparum. | Zhao Y, Kappes B, Yang J, Franklin RM | 10.1111/j.1432-1033.1992.tb17051.x |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18762219 | Characterization of Plasmodium falciparum protein kinase 2. | Kato K, Sudo A, ..., Akashi H | 10.1016/j.molbiopara.2008.07.007 |
| 37121260 | PK1 from Drosophila obscurin is an inactive pseudokinase with scaffolding properties | Zacharchenko T, Dorendorf T, ..., Mayans O | 10.1098/rsob.220350 |