PF3D7_1239000

Genome location: Pf3D7_12_v3:1,620,278..1,625,196(-)

Genome classification: Core

Function and Localization

Product Description: HD superfamily phosphohydrolase protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.4.17 (3',5'-cyclic-nucleotide phosphodiesterase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0008832	dGTPase activity
Process	GO:0006203	dGTP catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.14	0.93
Trophozoite	expressed	0.25	0.85
Schizont	expressed	0.61	0.66
Gametocyte	expressed	0.42	0.76

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1239000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.351 | #Insertions: 4

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I533

"Best" AlphaFill ligand hit: DGT (2'-deoxyguanosine-5'-triphosphate, Local RMSD=0.03) with 4MZ7 (Global RMSD=5.92)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.4.17	3',5'-cyclic-nucleotide phosphodiesterase	ATPADPFADAMPGTPGDPGMPNaFNAD+NADHEDTAcAMP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100615

Most Similar Human Ortholog: A0A2R8YCS7

TM-align score: 0.73 | RMSD: 3.47

Seq Identity: 0.30 | Length: 472 / 822

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000101347	SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	45	34	58
disruptive	11	50	50	93
missense	7	45	47	79

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	19	95	42	58
disruptive	24	150	81	220
missense	14	122	72	145

PlasmoDB Total SNPs: 257

Non-coding: 194 | Synonymous: 48 | Nonsynonymous: 15 | Stop Codon: 0

Protein Information

Protein Length: 822 | Molecular Weight (kDa): 96.005

UniProt ID(s): Q8I533

PDB ID(s): None

Isoelectric Point: 7.51

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003607	HD/PDEase domain
InterPro	IPR006674	HD domain
PFam	PF01966	HD domain
Superfamily	SSF109604	N/A