Genome location: Pf3D7_12_v3:1,652,687..1,654,402(-)
Genome classification: Core
Product Description: hydroxyethylthiazole kinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.50 (Hydroxyethylthiazole kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0004417 | hydroxyethylthiazole kinase activity |
| Process | GO:0009228 | thiamine biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.14 | 0.91 |
| Schizont | expressed | 0.03 | 0.98 |
| Gametocyte | possibly expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1239600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.969 | MFS: -2.164 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I527
"Best" AlphaFill ligand hit: TZE (2-(4-methyl-thiazol-5-yl)-ethanol, Local RMSD=0.56) with 1C3Q (Global RMSD=4.17)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.50 | hydroxyethylthiazole kinase | PCMB2-Aminohydroxyethylthiazole |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 16 | 10 | 15 |
| disruptive | 5 | 25 | 19 | 39 |
| missense | 5 | 25 | 19 | 36 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 33 | 12 | 20 |
| disruptive | 11 | 55 | 37 | 66 |
| missense | 11 | 50 | 37 | 44 |
PlasmoDB Total SNPs: 49
Non-coding: 36 | Synonymous: 6 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 302 | Molecular Weight (kDa): 33.882
PDB ID(s): None
Isoelectric Point: 8.76
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000417 | Hydroxyethylthiazole kinase |
| InterPro | IPR029056 | Ribokinase-like |
| PFam | PF02110 | Hydroxyethylthiazole kinase |
| Superfamily | SSF53613 | Ribokinase-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368868 | Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14. | Gardner MJ, Shallom SJ, ..., Fraser CM | 10.1038/nature01094 |
| 26049116 | Isolation, characterization, interaction of a thiazolekinase (Plasmodiumfalciparum) with silver nanoparticles. | Yao J, van Marwijk J, Wilhelmi B, Whiteley CG | 10.1016/j.ijbiomac.2015.05.033 |