PF3D7_1239900 (VPS16)

Genome location: Pf3D7_12_v3:1,678,397..1,682,107(-)

Genome classification: Core

Function and Localization

Product Description: vacuolar protein sorting-associated protein 16, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0030897	HOPS complex
Component	GO:0005768	endosome
Function	GO:0003779	actin binding
Process	GO:0016197	endosomal transport
Process	GO:0035542	regulation of SNARE complex assembly
Process	GO:0032889	regulation of vacuole fusion, non-autophagic

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.34	0.83
Trophozoite	possibly expressed	0.40	0.76
Schizont	possibly expressed	0.14	0.91
Gametocyte	possibly expressed	0.44	0.74

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1239900

Resistome Missense Mutations: N311K

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.026 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1454300)

Relative Growth Rate: 0.04 ± 0.28
Confidence: 3.92

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I524

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102133

Most Similar Human Ortholog: Q5JUB0

TM-align score: 0.78 | RMSD: 2.71

Seq Identity: 0.19 | Length: 209 / 1032

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000215305	VPS16 core subunit of CORVET and HOPS complexes

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	18	42	12	43
disruptive	23	81	46	116
missense	7	64	37	88

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	30	68	23	56
disruptive	53	164	80	212
missense	21	122	63	132

PlasmoDB Total SNPs: 167

Non-coding: 78 | Synonymous: 73 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 1032 | Molecular Weight (kDa): 120.3

UniProt ID(s): Q8I524

PDB ID(s): None

Isoelectric Point: 8.62

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006925	Vps16, C-terminal
InterPro	IPR006926	Vps16, N-terminal
InterPro	IPR016534	Vacuolar protein sorting-associated protein 16
PFam	PF04840	Vps16, C-terminal
PFam	PF04841	Vps16, N-terminal
Superfamily	N/A	N/A