Product Description: 3-hydroxyisobutyryl-CoA hydrolase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.2.4 (3-hydroxyisobutyryl-CoA hydrolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0003860 | 3-hydroxyisobutyryl-CoA hydrolase activity |
| Process | GO:0006574 | valine catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.17 | 0.91 |
| Trophozoite | not expressed | 0.06 | 0.96 |
| Schizont | not expressed | 0.03 | 0.98 |
| Gametocyte | possibly expressed | 0.26 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1240000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.96 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1454400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I523
"Best" AlphaFill ligand hit: ACO (acetyl coenzyme *a, Local RMSD=0.20) with 1WDM (Global RMSD=3.99)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.2.4 | 3-hydroxyisobutyryl-CoA hydrolase | acetyl-CoAmalonyl-CoApropionyl-CoAn-valeryl-CoAisobutyryl-CoAacetoacetyl-CoADL-methylmalonyl-CoADL-3-hydroxybutyryl-CoA |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102025
Most Similar Human Ortholog: Q6NVY1
TM-align score: 0.83 | RMSD: 2.57
Seq Identity: 0.25 | Length: 350 / 541
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000198130 | 3-hydroxyisobutyryl-CoA hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 12 | 4 | 15 |
| disruptive | 11 | 38 | 14 | 60 |
| missense | 9 | 38 | 14 | 50 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 23 | 11 | 20 |
| disruptive | 18 | 69 | 40 | 95 |
| missense | 14 | 61 | 37 | 65 |
PlasmoDB Total SNPs: 107
Non-coding: 66 | Synonymous: 31 | Nonsynonymous: 10 | Stop Codon: 0
Protein Length: 541 | Molecular Weight (kDa): 63.856
UniProt ID(s): Q8I523
PDB ID(s): None
Isoelectric Point: 9.08
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR029045 | ClpP/crotonase-like domain superfamily |
| InterPro | IPR032259 | Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type |
| PFam | PF16113 | Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type |
| Superfamily | SSF52096 | ClpP/crotonase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |