PF3D7_1240900 (VAR)

Genome location: Pf3D7_12_v3:1,734,992..1,744,191(+)

Genome classification: InternalHypervariable

Function and Localization

Product Description: erythrocyte membrane protein 1, PfEMP1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.03	0.99
Trophozoite	expressed	0.09	0.94
Schizont	expressed	0.20	0.86
Gametocyte	expressed	0.36	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1240900

Resistome Missense Mutations: K289N, P293A, K294M, A300P, H560R, D657N, E658D, Y668F, E725D, D1063H, A1825S, V1887I

Resistome Compounds with Missense Mutations: AN13762, AN13956, Halofuginone, Pepstatin n-butyl ester

Resistome # Samples with Disruptive Mutations: 16 (16 missense, 10 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.006 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A1K0

"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=2.84) with 5MZA (Global RMSD=6.46)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104345

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 3181

Non-coding: 706 | Synonymous: 580 | Nonsynonymous: 1858 | Stop Codon: 37

Protein Information

Protein Length: 2287 | Molecular Weight (kDa): 258.476

UniProt ID(s): A0A144A1K0, A0A5P1A038, A0A5P1A044, A0A5P1A0B3, A0A5P1A0E2, A0A5P1A0P1, A0A5P1A2F3, A3R6U2, O60991

PDB ID(s): None

Isoelectric Point: 5.12

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004258	Duffy-binding-like domain
InterPro	IPR008602	Duffy-antigen binding
InterPro	IPR029210	Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment
InterPro	IPR029211	Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment
InterPro	IPR041480	Cysteine-rich interdomain region 1 gamma
PFam	PF03011	Duffy-binding-like domain
PFam	PF05424	Duffy-antigen binding
PFam	PF15445	Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment
PFam	PF15447	Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment
PFam	PF18562	Cysteine-rich interdomain region 1 gamma
Superfamily	SSF140924	N/A