PF3D7_1241100 (PFS2)

Genome location: Pf3D7_12_v3:1,746,049..1,748,494(-)

Genome classification: Core

Function and Localization

Product Description: polyadenylation factor subunit 2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005847	mRNA cleavage and polyadenylation specificity factor complex
Process	GO:0006379	mRNA cleavage
Process	GO:0006378	mRNA polyadenylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.36	0.82
Trophozoite	expressed	0.21	0.87
Schizont	expressed	0.12	0.92
Gametocyte	expressed	0.12	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1241100

Resistome Missense Mutations: D455E

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.18 | MFS: -2.897 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I518

"Best" AlphaFill ligand hit: SIW (keggin (sta), Local RMSD=0.02) with 6Y7O (Global RMSD=4.63)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102397

Most Similar Human Ortholog: C9J8B4

TM-align score: 0.83 | RMSD: 2.35

Seq Identity: 0.24 | Length: 188 / 468

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000136709	WD repeat domain 33

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	9	11	26
disruptive	4	16	15	44
missense	3	14	11	38

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	33	20	13
disruptive	11	40	26	83
missense	5	31	22	59

PlasmoDB Total SNPs: 84

Non-coding: 54 | Synonymous: 20 | Nonsynonymous: 9 | Stop Codon: 1

Protein Information

Protein Length: 468 | Molecular Weight (kDa): 53.559

UniProt ID(s): Q8I518

PDB ID(s): None

Isoelectric Point: 9.28

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR019775	WD40 repeat, conserved site
InterPro	IPR036322	WD40-repeat-containing domain superfamily
PFam	PF00400	WD40 repeat
Superfamily	SSF50978	WD40-repeat-containing domain superfamily