Genome location: Pf3D7_12_v3:1,777,080..1,779,980(+)
Genome classification: Core
Product Description: replication factor C subunit 4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005663 | DNA replication factor C complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0003689 | DNA clamp loader activity |
| Process | GO:0006281 | DNA repair |
| Process | GO:0006260 | DNA replication |
| Process | GO:0006261 | DNA-dependent DNA replication |
| Process | GO:0006272 | leading strand elongation |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.37 | 0.77 |
| Schizont | expressed | 0.05 | 0.96 |
| Gametocyte | expressed | 0.21 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1241700
Resistome Missense Mutations: S42T
Resistome Compounds with Missense Mutations: ACT-451840
Resistome # Samples with Disruptive Mutations: 3 (3 missense, 3 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.256 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1455100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1455100)
Modification: Disrupted | RMgm-4015
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I512
"Best" AlphaFill ligand hit: 08T ([[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium, Local RMSD=0.02) with 3U5Z (Global RMSD=7.27)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P04526 | Sliding-clamp-loader large/small subunit | OrthoMCL BLAST | d5-NITPd6-NITP |
Ortholog Group (OrthoMCL): OG6_100321
Most Similar Human Ortholog: P35250
TM-align score: 0.93 | RMSD: 1.92
Seq Identity: 0.47 | Length: 326 / 336
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000049541 | replication factor C subunit 2 |
| ENSG00000111445 | replication factor C subunit 5 |
Protein Length: 336 | Molecular Weight (kDa): 37.638
PDB ID(s): None
Isoelectric Point: 7.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR008921 | DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00004 | ATPase, AAA-type, core |
| Superfamily | SSF48019 | DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 22 | 12 | 16 |
| disruptive | 1 | 5 | 5 | 19 |
| missense | 1 | 5 | 4 | 16 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 35 | 19 | 15 |
| disruptive | 4 | 17 | 28 | 67 |
| missense | 3 | 12 | 23 | 36 |
PlasmoDB Total SNPs: 135
Non-coding: 114 | Synonymous: 21 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 29326268 | Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics. | Cowell AN, Istvan ES, ..., Winzeler EA | 10.1126/science.aan4472 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33040440 | Plasmodium falciparum replication factor C subunit 1 is involved in genotoxicstress response. | Sheriff O, Yaw A, ..., Preiser PR | 10.1111/cmi.13277 |
| 35483337 | Integrative analysis of expression, prognostic significance and immuneinfiltration of RFC family genes in human sarcoma | Wu G, Zhou J, ..., Wu T | 10.18632/aging.204039 |