PF3D7_1241800 (DBP9)

Genome location: Pf3D7_12_v3:1,779,442..1,783,743(-)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase DBP9, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0043186	P granule
Component	GO:0005634	nucleus
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity
Process	GO:0016070	RNA metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.12	0.51
Trophozoite	expressed	0.77	0.59
Schizont	expressed	0.34	0.79
Gametocyte	expressed	0.47	0.74

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1241800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -3.034 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1455200)

Relative Growth Rate: 0.18 ± 0.22
Confidence: 4.44

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I511

"Best" AlphaFill ligand hit: KEG (12-tungstophosphate, Local RMSD=0.59) with 5SUQ (Global RMSD=6.10)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102730

Most Similar Human Ortholog: F8WDT8

TM-align score: 0.86 | RMSD: 1.98

Seq Identity: 0.29 | Length: 176 / 878

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000136271	DEAD-box helicase 56

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	35	33	51
disruptive	62	190	103	183
missense	33	93	70	125

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	14	80	34	59
disruptive	122	335	131	218
missense	52	199	92	131

PlasmoDB Total SNPs: 234

Non-coding: 22 | Synonymous: 70 | Nonsynonymous: 142 | Stop Codon: 0

Protein Information

Protein Length: 878 | Molecular Weight (kDa): 103.108

UniProt ID(s): Q8I511

PDB ID(s): None

Isoelectric Point: 4.88

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
36761420	Synthetic lethal interactions of DEAD/H-box helicases as targets for cancertherapy	Arna AB, Patel H, ..., Wu Y	10.3389/fonc.2022.1087989
35139378	A comprehensive landscape of 60S ribosome biogenesis factors	Sailer C, Jansen J, ..., Stengel F	10.1016/j.celrep.2022.110353
15028736	Dbp9p, a member of the DEAD box protein family, exhibits DNA helicase activity	Kikuma T, Ohtsu M, ..., Murakami Y	10.1074/jbc.M400231200
16449635	Comprehensive mutational analysis of yeast DEXD/H box RNA helicases involved inlarge ribosomal subunit biogenesis	Bernstein KA, Granneman S, ..., Baserga SJ	10.1128/MCB.26.4.1195-1208.2006
11565753	Dbp9p, a putative ATP-dependent RNA helicase involved in 60S-ribosomal-subunitbiogenesis, functionally interacts with Dbp6p	Daugeron MC, Kressler D, Linder P	10.1017/s1355838201010640