Genome location: Pf3D7_12_v3:1,786,955..1,788,989(-)
Genome classification: Core
Product Description: sulfhydryl oxidase, putative
SignalP Peptide: MKVTYAVRLLLPVYFLFFWIHLNIVRS
# Transmembrane Domains: 1
EC Numbers: 1.8.3.2 (Thiol oxidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005615 | extracellular space |
| Component | GO:0030173 | integral component of Golgi membrane |
| Function | GO:0016971 | flavin-linked sulfhydryl oxidase activity |
| Function | GO:0003756 | protein disulfide isomerase activity |
| Function | GO:0016972 | thiol oxidase activity |
| Process | GO:0006457 | protein folding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.98 |
| Trophozoite | not expressed | 0.15 | 0.90 |
| Schizont | not expressed | 0.14 | 0.90 |
| Gametocyte | not expressed | 0.53 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1242000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.01 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1455400)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1455400)
Modification: Disrupted | RMgm-4855
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I509
"Best" AlphaFill ligand hit: FAD (flavin-adenine dinucleotide, Local RMSD=4.19) with 3QCP (Global RMSD=5.04)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.8.3.2 | thiol oxidase | KINEMKCNDTTEDTAGSSGRNAiazideZnSO4MgSO4Na2SO4guanine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102428
Most Similar Human Ortholog: Q6ZRP7
TM-align score: 0.60 | RMSD: 4.29
Seq Identity: 0.15 | Length: 383 / 531
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000116260 | quiescin sulfhydryl oxidase 1 |
| ENSG00000165661 | quiescin sulfhydryl oxidase 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 18 | 14 | 28 |
| disruptive | 20 | 31 | 26 | 44 |
| missense | 16 | 28 | 24 | 41 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 45 | 23 | 26 |
| disruptive | 24 | 82 | 50 | 108 |
| missense | 19 | 68 | 46 | 68 |
PlasmoDB Total SNPs: 84
Non-coding: 51 | Synonymous: 18 | Nonsynonymous: 14 | Stop Codon: 1
Protein Length: 531 | Molecular Weight (kDa): 63.727
UniProt ID(s): Q8I509
PDB ID(s): None
Isoelectric Point: 7.11
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR017905 | ERV/ALR sulfhydryl oxidase domain |
| InterPro | IPR036249 | Thioredoxin-like superfamily |
| InterPro | IPR036774 | ERV/ALR sulfhydryl oxidase domain superfamily |
| InterPro | IPR039798 | Sulfhydryl oxidase |
| PFam | PF04777 | ERV/ALR sulfhydryl oxidase domain |
| Superfamily | SSF52833 | Thioredoxin-like superfamily |
| Superfamily | SSF69000 | ERV/ALR sulfhydryl oxidase domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 32670896 | Characterization of a Sulfhydryl Oxidase From Plasmodium berghei as a Target forBlocking Parasite Transmission. | Zheng W, Liu F, ..., Cao Y | 10.3389/fcimb.2020.00311 |
| 34844425 | The Spliced Leader RNA Silencing (SLS) Pathway in Trypanosoma brucei Is Inducedby Perturbations of Endoplasmic Reticulum, Golgi Complex, or MitochondrialProtein Factors: Functional Analysis of SLS-Inducing Kinase PK3 | Okalang U, Mualem Bar-Ner B, ..., Michaeli S | 10.1128/mBio.02602-21 |