Genome location: Pf3D7_12_v3:1,791,156..1,793,341(+)
Genome classification: Core
Product Description: queuine tRNA-ribosyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.4.2.29 (tRNA-guanine(34) transglycosylase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0008479 | queuine tRNA-ribosyltransferase activity |
| Process | GO:0002099 | tRNA wobble guanine modification |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.09 | 0.95 |
| Trophozoite | expressed | 0.24 | 0.85 |
| Schizont | expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.33 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1242200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.147 | MFS: -2.829 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0615100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I507
"Best" AlphaFill ligand hit: TEW (6-tungstotellurate(vi), Local RMSD=0.26) with 7OVS (Global RMSD=4.89)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.2.29 | tRNA-guanosine34 preQ1 transglycosylase | ethylacetimidate4-aminophthalimidedimethylsuberimidate4-aminophthalhydrazide3,5-diaminophthalimide2,3-Dihydroxybenzoic acid2-aminopteridin-4(3H)-one3,5-diaminophthalhydrazide2-aminoquinazolin-4(3H)-onebenzimidazole-5-carboxylate2-methylamino-lin-benzoguanine2,6-diaminoquinazolin-4(3H)-one... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9BXR0 | Queuine tRNA-ribosyltransferase catalytic subunit 1 | Homo sapiens | OrthoDB | CHEMBL6993CHEMBL487739 |
Ortholog Group (OrthoMCL): OG6_100602
Most Similar Human Ortholog: Q9BXR0
TM-align score: 0.89 | RMSD: 1.92
Seq Identity: 0.33 | Length: 378 / 639
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000213339 | queuine tRNA-ribosyltransferase catalytic subunit 1 |
Protein Length: 639 | Molecular Weight (kDa): 74.945
UniProt ID(s): Q8I507
PDB ID(s): None
Isoelectric Point: 10
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002616 | tRNA-guanine(15) transglycosylase-like |
| InterPro | IPR036511 | Queuine tRNA-ribosyltransferase-like |
| PFam | PF01702 | tRNA-guanine(15) transglycosylase-like |
| Superfamily | SSF51713 | Queuine tRNA-ribosyltransferase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 30 | 18 | 53 |
| disruptive | 14 | 69 | 44 | 101 |
| missense | 14 | 61 | 36 | 82 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 74 | 38 | 45 |
| disruptive | 35 | 154 | 72 | 180 |
| missense | 27 | 123 | 52 | 117 |
PlasmoDB Total SNPs: 53
Non-coding: 9 | Synonymous: 20 | Nonsynonymous: 23 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 25869381 | Identification of Plasmodium falciparum apicoplast-targeted tRNA-guaninetransglycosylase and its potential inhibitors using comparative genomics,molecular modelling, docking and simulation studies. | Sawhney B, Chopra K, Misra R, Ranjan A | 10.1080/07391102.2015.1040074 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 37932470 | Aotus nancymaae model predicts human immune response to the placental malariavaccine candidate VAR2CSA | Doritchamou J, Nielsen MA, ..., Duffy PE | 10.1038/s41684-023-01274-2 |