PF3D7_1242600

Genome location: Pf3D7_12_v3:1,800,215..1,802,786(+)

Genome classification: Core

Function and Localization

Product Description: protein farnesyltransferase subunit alpha

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.5.1.58 (Protein farnesyltransferase);2.5.1.60 (Protein geranylgeranyltransferase type II)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005953	CAAX-protein geranylgeranyltransferase complex
Component	GO:0005737	cytoplasm
Component	GO:0005965	protein farnesyltransferase complex
Process	GO:0018343	protein farnesylation
Process	GO:0018344	protein geranylgeranylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.19	0.91
Trophozoite	expressed	0.32	0.81
Schizont	expressed	0.04	0.97
Gametocyte	possibly expressed	0.21	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1242600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.101 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1456000)

Relative Growth Rate: 0.04 ± 0.10
Confidence: 5.97

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I503

"Best" AlphaFill ligand hit: GRG (geranylgeranyl diphosphate, Local RMSD=0.10) with 1N4P (Global RMSD=2.00)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.5.1.58	protein farnesyltransferase	ED5EDTACVFMCVIMDPI-1FTI-276R115777FTI-277KTSCVAMKTSCVFMKTSCVIAKTSCVIF...
2.5.1.60	protein geranylgeranyltransferase type II	BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q04631	Protein farnesyltransferase subunit beta/geranylgeranyltransferase type-1 subunit alpha	Rattus norvegicus	OrthoDB	Chem Biol Compound 2Sulfonamide 12bSulfonamide 17Sulfonamide 12aa...
Q61239	Protein farnesyltransferase subunit beta/geranylgeranyltransferase type-1 subunit alpha	Mus musculus	OrthoDB	CHEMBL158553A345677CHEMBL29633CHEMBL280847...
P49354	Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha	Homo sapiens	OrthoDB	A313326 Analogue 7A313326 Analogue 8A313326 Analogue 9A313326 Analogue 10...
P29703	Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha	Saccharomyces cerevisiae	OrthoDB, OrthoMCL	CHEMBL573337CHEMBL573337CHEMBL572404CHEMBL572404...
P29702	Protein farnesyltransferase subunit beta/geranylgeranyltransferase type-1 subunit alpha	Bos taurus	OrthoDB	Compound ICompound IICompound IIICompound IV...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102620

Most Similar Human Ortholog: E9PQP6

TM-align score: 0.91 | RMSD: 2.16

Seq Identity: 0.22 | Length: 248 / 521

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000168522	farnesyltransferase, CAAX box, alpha

Protein Information

Protein Length: 521 | Molecular Weight (kDa): 63.854

UniProt ID(s): A0FDU3, Q5EI73, Q5EI75, Q8I503

PDB ID(s): None

Isoelectric Point: 5.16

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002088	Protein prenyltransferase, alpha subunit
PFam	PF01239	Protein prenyltransferase, alpha subunit
Superfamily	SSF48439	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	28	16	25
disruptive	16	82	43	118
missense	16	81	41	112

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	51	31	31
disruptive	34	171	75	149
missense	29	158	64	118

PlasmoDB Total SNPs: 125

Non-coding: 86 | Synonymous: 14 | Nonsynonymous: 25 | Stop Codon: 0