Genome location: Pf3D7_12_v3:1,852,659..1,857,347(-)
Genome classification: Core
Product Description: RNA polymerase II transcription factor B subunit 2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0000439 | transcription factor TFIIH core complex |
| Component | GO:0005675 | transcription factor TFIIH holo complex |
| Function | GO:0003690 | double-stranded DNA binding |
| Process | GO:0006289 | nucleotide-excision repair |
| Process | GO:0070816 | phosphorylation of RNA polymerase II C-terminal domain |
| Process | GO:0006366 | transcription by RNA polymerase II |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.18 | 0.91 |
| Trophozoite | expressed | 0.32 | 0.80 |
| Schizont | possibly expressed | 0.10 | 0.94 |
| Gametocyte | not expressed | 0.21 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1244200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.01 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1457500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4Y8
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101881
Most Similar Human Ortholog: A0A0G2JHJ2
TM-align score: 0.75 | RMSD: 2.57
Seq Identity: 0.17 | Length: 122 / 945
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000213780 | general transcription factor IIH subunit 4 |
| ENSG00000221974 | general transcription factor IIH subunit 4 |
| ENSG00000226384 | general transcription factor IIH subunit 4 |
| ENSG00000233149 | general transcription factor IIH subunit 4 |
| ENSG00000234370 | general transcription factor IIH subunit 4 |
| ENSG00000236895 | general transcription factor IIH subunit 4 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 36 | 21 | 63 |
| disruptive | 58 | 104 | 52 | 136 |
| missense | 33 | 85 | 42 | 117 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 75 | 43 | 86 |
| disruptive | 72 | 216 | 103 | 195 |
| missense | 43 | 163 | 84 | 142 |
PlasmoDB Total SNPs: 303
Non-coding: 160 | Synonymous: 50 | Nonsynonymous: 93 | Stop Codon: 0
Protein Length: 945 | Molecular Weight (kDa): 111.87
UniProt ID(s): Q8I4Y8
PDB ID(s): None
Isoelectric Point: 4.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004598 | Transcription factor TFIIH subunit p52/Tfb2 |
| InterPro | IPR040662 | Transcription factor Tfb2, C-terminal domain |
| PFam | PF03849 | Transcription factor TFIIH subunit p52/Tfb2 |
| PFam | PF18307 | Transcription factor Tfb2, C-terminal domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 19897425 | Interacting partners of the Tfb2 subunit from yeast TFIIH | Kainov DE, Selth LA, ..., Poterzsman A | 10.1016/j.dnarep.2009.10.002 |
| 27381459 | Function of Conserved Topological Regions within the Saccharomyces cerevisiaeBasal Transcription Factor TFIIH | Warfield L, Luo J, Ranish J, Hahn S | 10.1128/MCB.00182-16 |
| 20606254 | Structure determination of the minimal complex between Tfb5 and Tfb2, twosubunits of the yeast transcription/DNA-repair factor TFIIH: a retrospectivestudy | Kainov DE, Cura V, ..., Poterszman A | 10.1107/S0907444910009844 |