PF3D7_1245000

Genome location: Pf3D7_12_v3:1,879,903..1,881,465(-)

Genome classification: Core

Function and Localization

Product Description: 5-formyltetrahydrofolate cyclo-ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.3.3.2 (5-formyltetrahydrofolate cyclo-ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0030272	5-formyltetrahydrofolate cyclo-ligase activity
Process	GO:0009396	folic acid-containing compound biosynthetic process
Process	GO:0035999	tetrahydrofolate interconversion
Process	GO:0046653	tetrahydrofolate metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.09	0.96
Trophozoite	not expressed	0.39	0.77
Schizont	expressed	0.25	0.84
Gametocyte	expressed	1.14	0.45

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1245000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.624 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I4Y1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.3.3.2	5-formyltetrahydrofolate cyclo-ligase	AMPNEMSO42-AgNO3MgADP-oxalateMaleateLY309887phosphatesuccinate(NH4)2SO4pemetrexed...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101474

Most Similar Human Ortholog: P49914

TM-align score: 0.82 | RMSD: 2.86

Seq Identity: 0.18 | Length: 197 / 456

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000136371	methenyltetrahydrofolate synthetase
ENSG00000259332	ST20-MTHFS readthrough

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	12	10	28
disruptive	9	53	39	68
missense	9	53	38	64

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	33	21	33
disruptive	21	119	57	113
missense	17	104	54	85

PlasmoDB Total SNPs: 33

Non-coding: 5 | Synonymous: 18 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 456 | Molecular Weight (kDa): 55.268

UniProt ID(s): Q8I4Y1

PDB ID(s): None

Isoelectric Point: 7.99

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002698	5-formyltetrahydrofolate cyclo-ligase
InterPro	IPR037171	NagB/RpiA transferase-like
PFam	PF01812	5-formyltetrahydrofolate cyclo-ligase
Superfamily	SSF100950	NagB/RpiA transferase-like