Genome location: Pf3D7_12_v3:1,882,683..1,888,206(+)
Genome classification: Core
Product Description: kinesin-13, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005871 | kinesin complex |
| Component | GO:0000776 | kinetochore |
| Component | GO:0005634 | nucleus |
| Function | GO:0003774 | cytoskeletal motor activity |
| Process | GO:0035082 | axoneme assembly |
| Process | GO:0007018 | microtubule-based movement |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.31 | 0.86 |
| Trophozoite | possibly expressed | 0.51 | 0.70 |
| Schizont | expressed | 0.26 | 0.84 |
| Gametocyte | expressed | 0.83 | 0.55 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1245100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.99 | MFS: -2.663 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1458300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1458300)
Modification: Tagged | RMgm-5020
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4Y0
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.08) with 6TRL (Global RMSD=5.69)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8NI77 | Kinesin-like protein KIF18A | OrthoDB | US11236069, Example 1US11236069, Example 1-1US11236069, Example 1-2US11236069, Example 1-3... |
Ortholog Group (OrthoMCL): OG6_100492
Most Similar Human Ortholog: D6R9M0
TM-align score: 0.55 | RMSD: 2.66
Seq Identity: 0.38 | Length: 306 / 1326
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000068796 | kinesin family member 2A |
| ENSG00000141200 | kinesin family member 2B |
| ENSG00000142945 | kinesin family member 2C |
| ENSG00000186638 | kinesin family member 24 |
| ENSG00000288643 | novel transcript |
Protein Length: 1326 | Molecular Weight (kDa): 153.126
UniProt ID(s): Q8I4Y0
PDB ID(s): 1RY6
Isoelectric Point: 7.89
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001752 | Kinesin motor domain |
| InterPro | IPR019821 | Kinesin motor domain, conserved site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR027640 | Kinesin-like protein |
| PFam | PF00225 | Kinesin motor domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 64 | 47 | 94 |
| disruptive | 80 | 156 | 73 | 250 |
| missense | 37 | 105 | 54 | 191 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 34 | 145 | 53 | 110 |
| disruptive | 123 | 340 | 183 | 431 |
| missense | 58 | 234 | 138 | 286 |
PlasmoDB Total SNPs: 316
Non-coding: 167 | Synonymous: 82 | Nonsynonymous: 67 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 35900985 | Genome-wide functional analysis reveals key roles for kinesins in the mammalianand mosquito stages of the malaria parasite life cycle. | Zeeshan M, Rashpa R, ..., Tewari R | 10.1371/journal.pbio.3001704 |