PF3D7_1245300 (UBC12)

Genome location: Pf3D7_12_v3:1,892,918..1,894,243(+)

Genome classification: Core

Function and Localization

Product Description: NEDD8-conjugating enzyme UBC12, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0019788	NEDD8 transferase activity
Function	GO:0061631	ubiquitin conjugating enzyme activity
Function	GO:0004842	ubiquitin-protein transferase activity
Process	GO:0045116	protein neddylation
Process	GO:0000209	protein polyubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	expressed	0.03	0.98
Schizont	possibly expressed	0.00	1.00
Gametocyte	not expressed	0.02	0.99

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1245300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.124 | MFS: -3.087 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I4X8

"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.68) with 4FH1 (Global RMSD=2.82)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.3.2.19	ubiquitin-protein ligase	No BRENDA inhibitors

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q969M7	NEDD8-conjugating enzyme UBE2F		OrthoDB	US9963456, Example 1US9963456, Example 2US10174040, Example 8US9963456, Example 10...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102346

Most Similar Human Ortholog: M0QX69

TM-align score: 0.90 | RMSD: 1.01

Seq Identity: 0.43 | Length: 69 / 157

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000130725	ubiquitin conjugating enzyme E2 M

Protein Information

Protein Length: 157 | Molecular Weight (kDa): 18.401

UniProt ID(s): Q8I4X8

PDB ID(s): None

Isoelectric Point: 4.6

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000608	Ubiquitin-conjugating enzyme E2
InterPro	IPR016135	Ubiquitin-conjugating enzyme/RWD-like
InterPro	IPR023313	Ubiquitin-conjugating enzyme, active site
PFam	PF00179	Ubiquitin-conjugating enzyme E2
Superfamily	SSF54495	Ubiquitin-conjugating enzyme/RWD-like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	10	2	9
disruptive	0	9	4	10
missense	0	9	4	9

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	15	5	10
disruptive	0	17	8	29
missense	0	16	6	23

PlasmoDB Total SNPs: 96

Non-coding: 87 | Synonymous: 8 | Nonsynonymous: 1 | Stop Codon: 0