PF3D7_1245600

Genome location: Pf3D7_12_v3:1,900,249..1,905,066(-)

Genome classification: Core

Function and Localization

Product Description: kinesin-15, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.09	0.95
Trophozoite	possibly expressed	0.04	0.98
Schizont	possibly expressed	0.03	0.98
Gametocyte	expressed	0.68	0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1245600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.509 | #Insertions: 3

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1458800)

Relative Growth Rate: 0.07 ± 0.17
Confidence: 4.92

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1458800)

Modification: Tagged | RMgm-5021

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I4X5

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.06) with 2GM1 (Global RMSD=4.76)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_215365

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	19	82	53	102
disruptive	184	270	119	243
missense	84	158	85	174

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	37	167	75	105
disruptive	256	468	219	452
missense	111	297	147	292

PlasmoDB Total SNPs: 275

Non-coding: 0 | Synonymous: 123 | Nonsynonymous: 151 | Stop Codon: 1

Protein Information

Protein Length: 1605 | Molecular Weight (kDa): 188.16

UniProt ID(s): Q8I4X5

PDB ID(s): None

Isoelectric Point: 7.7

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001752	Kinesin motor domain
InterPro	IPR019821	Kinesin motor domain, conserved site
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR027640	Kinesin-like protein
PFam	PF00225	Kinesin motor domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
35900985	Genome-wide functional analysis reveals key roles for kinesins in the mammalianand mosquito stages of the malaria parasite life cycle.	Zeeshan M, Rashpa R, ..., Tewari R	10.1371/journal.pbio.3001704