Genome location: Pf3D7_12_v3:1,919,898..1,923,323(+)
Genome classification: Core
Product Description: actin I
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005884 | actin filament |
| Component | GO:0009986 | cell surface |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0031941 | filamentous actin |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0098808 | mRNA cap binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0005200 | structural constituent of cytoskeleton |
| Process | GO:0070359 | actin polymerization-dependent cell motility involved in migration of symbiont in host |
| Process | GO:0051301 | cell division |
| Process | GO:0007010 | cytoskeleton organization |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.05 | 0.65 |
| Trophozoite | expressed | 1.53 | 0.32 |
| Schizont | expressed | 3.42 | 0.02 |
| Gametocyte | expressed | 2.33 | 0.16 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1246200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.213 | MFS: -2.745 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1459300)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1459300)
Modification: Mutated | RMgm-5230
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4X0
"Best" AlphaFill ligand hit: ACO (acetyl coenzyme *a, Local RMSD=0.07) with 6NAS (Global RMSD=0.98)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P60010 | Actin [R335K] | HOGENOM, OMA, OrthoDB | SCHEMBL1889389Latrunculin ASCHEMBL1889389Latrunculin A... |
Ortholog Group (OrthoMCL): OG6_100127
Most Similar Human Ortholog: P68032
TM-align score: 1.00 | RMSD: 0.44
Seq Identity: 0.81 | Length: 376 / 376
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000075624 | actin beta |
| ENSG00000107796 | actin alpha 2, smooth muscle |
| ENSG00000143632 | actin alpha 1, skeletal muscle |
| ENSG00000159251 | actin alpha cardiac muscle 1 |
| ENSG00000163017 | actin gamma 2, smooth muscle |
| ENSG00000166351 | POTE ankyrin domain family member D |
| ENSG00000169067 | actin beta like 2 |
| ENSG00000183206 | POTE ankyrin domain family member C |
| ENSG00000184009 | actin gamma 1 |
| ENSG00000187537 | POTE ankyrin domain family member G |
| ENSG00000188219 | POTE ankyrin domain family member E |
| ENSG00000196604 | POTE ankyrin domain family member F |
| ENSG00000196834 | POTE ankyrin domain family member I |
| ENSG00000198062 | POTE ankyrin domain family member H |
| ENSG00000222036 | POTE ankyrin domain family member M |
| ENSG00000222038 | POTE ankyrin domain family member J |
| ENSG00000230031 | POTE ankyrin domain family member B2 |
| ENSG00000233917 | POTE ankyrin domain family member B |
| ENSG00000276760 | POTE ankyrin domain family member B-like |
| ENSG00000277630 | POTE ankyrin domain family member D-like |
| ENSG00000278522 | POTE ankyrin domain family member B3 |
| ENSG00000278699 | POTE ankyrin domain family member B3 |
| ENSG00000288113 | POTE ankyrin domain family member B2 |
| ENSG00000288142 | POTE ankyrin domain family member B |
| ENSG00000288347 | POTE ankyrin domain family member B |
Protein Length: 376 | Molecular Weight (kDa): 41.871
UniProt ID(s): A0A144A1R5, A4URS5, Q8I4X0, Q9U6U4
PDB ID(s): 5MVV, 6I4D, 6I4E, 6I4F, 6I4G, 6I4H, 6I4I, 6I4J, 6I4K, 6I4L, 6TU4, 6TU7, 7ALN
Isoelectric Point: 5
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004000 | Actin family |
| InterPro | IPR004001 | Actin, conserved site |
| InterPro | IPR020902 | Actin/actin-like conserved site |
| InterPro | IPR043129 | ATPase, nucleotide binding domain |
| PFam | PF00022 | Actin family |
| Superfamily | SSF53067 | ATPase, nucleotide binding domain |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 1 |
| disruptive | 0 | 0 | 0 | 1 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 205
Non-coding: 202 | Synonymous: 3 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15876372 | Malaria parasite actin filaments are very short. | Schmitz S, Grainger M, ..., Veigel C | 10.1016/j.jmb.2005.03.056 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 22100161 | A critical role of perinuclear filamentous actin in spatial repositioning andmutually exclusive expression of virulence genes in malaria parasites. | Zhang Q, Huang Y, ..., Scherf A | 10.1016/j.chom.2011.09.013 |
| 28759593 | Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodiumvivax salivary gland sporozoites. | Swearingen KE, Lindner SE, ..., Mikolajczak SA | https://pubmed.ncbi.nlm.nih.gov/28759593/ |
| 28810863 | Multiple essential functions of Plasmodium falciparum actin-1 during malariablood-stage development. | Das S, Lemgruber L, ..., Meissner M | 10.1186/s12915-017-0406-2 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 30599160 | Inner membrane complex 1l protein of Plasmodium falciparum links membrane lipidswith cytoskeletal element 'actin' and its associated motor 'myosin'. | Kumar V, Behl A, ..., Hora R | 10.1016/j.ijbiomac.2018.12.239 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 38070448 | Improved titer and stability of selenium nanoparticles produced by engineeredSaccharomyces cerevisiae | Sun J, Wang Y, ..., Wang Y | 10.1016/j.enzmictec.2023.110367 |
| 37991229 | Danielozyma pruni sp. nov., an asexual yeast species isolated from insect frass | Liu S, Guo QC, An ZR, Hui FL | 10.1099/ijsem.0.006124 |
| 37486335 | Hanseniaspora menglaensis f.a., sp. nov., a novel apiculate yeast speciesisolated from rotting wood | Chen X, Qiao YZ, Hui FL | 10.1099/ijsem.0.005970 |
| 38016605 | WLJP-025p, a homogeneous Lonicera japonica polysaccharide, attenuates atopicdermatitis by regulating the MAPK/NFkappaB/AP-1 axis via Act1 | Zhang T, Rao X, ..., Liu P | 10.1016/j.ijbiomac.2023.128435 |