Genome location: Pf3D7_12_v3:1,931,257..1,933,545(+)
Genome classification: Core
Product Description: myosin A-tail interacting protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0070258 | inner membrane pellicle complex |
| Function | GO:0017022 | myosin binding |
| Function | GO:0005515 | protein binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.70 | 0.76 |
| Trophozoite | expressed | 0.15 | 0.91 |
| Schizont | expressed | 1.08 | 0.52 |
| Gametocyte | expressed | 0.77 | 0.65 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1246400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.123 | MFS: -2.828 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1459500)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PY17X_1462100)
Modification: Tagged | RMgm-4499
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4W8
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 224
Non-coding: 212 | Synonymous: 4 | Nonsynonymous: 6 | Stop Codon: 2
Protein Length: 204 | Molecular Weight (kDa): 23.492
UniProt ID(s): Q8I4W8
PDB ID(s): 2QAC, 4AOM, 4GFT, 4MZJ, 4MZK, 4MZL, 4R1E, 6YCY, 6ZN3, 8A12, 8CDQ
Isoelectric Point: 3.93
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011992 | EF-hand domain pair |
| PFam | N/A | N/A |
| Superfamily | SSF47473 | EF-hand domain pair |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16337961 | The MTIP-myosin A complex in blood stage malaria parasites. | Green JL, Martin SR, ..., Holder AA | 10.1016/j.jmb.2005.11.027 |
| 16547135 | Structure of the MTIP-MyoA complex, a key component of the malaria parasiteinvasion motor. | Bosch J, Turley S, ..., Hol WG | 10.1073/pnas.0510907103 |
| 17628590 | The closed MTIP-myosin A-tail complex from the malaria parasite invasionmachinery. | Bosch J, Turley S, ..., Hol WG | 10.1016/j.jmb.2007.06.016 |
| 18768477 | The motor complex of Plasmodium falciparum: phosphorylation by acalcium-dependent protein kinase. | Green JL, Rees-Channer RR, ..., Holder AA | 10.1074/jbc.M803129200 |
| 19165333 | Rab11A-controlled assembly of the inner membrane complex is required forcompletion of apicomplexan cytokinesis. | Agop-Nersesian C, Naissant B, ..., Langsley G | 10.1371/journal.ppat.1000270 |
| 20174678 | Interaction and dynamics of the Plasmodium falciparum MTIP-MyoA complex, a keycomponent of the invasion motor in the malaria parasite. | Thomas JC, Green JL, ..., Tate EW | 10.1039/b922093c |
| 22932904 | Regulation of the Plasmodium motor complex: phosphorylation of myosin Atail-interacting protein (MTIP) loosens its grip on MyoA. | Douse CH, Green JL, ..., Cota E | 10.1074/jbc.M112.379842 |
| 30094538 | In vitro interaction between Plasmodium falciparum myosin B (PfMyoB) and myosin Atail interacting protein (MTIP). | Hernandez PC, Wasserman M, Chaparro-Olaya J | 10.1007/s00436-018-6039-8 |
| 31874192 | Polarization of MTIP is a signature of gliding locomotion in Plasmodium ookinetesand sporozoites | Siden-Kiamos I, Goosmann C, ..., Montagna GN | 10.1016/j.molbiopara.2019.111247 |
| 36250062 | Complementary crosstalk between palmitoylation and phosphorylation events in MTIPregulates its role during Plasmodium falciparum invasion | Anam Z, Kumari G, ..., Singh S | 10.3389/fcimb.2022.924424 |
| 28893907 | Compositional and expression analyses of the glideosome during the Plasmodiumlife cycle reveal an additional myosin light chain required for maximum motility | Green JL, Wall RJ, ..., Holder AA | 10.1074/jbc.M117.802769 |
| 33046215 | Full-length Plasmodium falciparum myosin A and essential light chain PfELCstructures provide new anti-malarial targets | Moussaoui D, Robblee JP, ..., Houdusse A | 10.7554/eLife.60581 |
| 32383851 | Peptide Probes for Plasmodium falciparum MyoA Tail Interacting Protein (MTIP):Exploring the Druggability of the Malaria Parasite Motor Complex | Saunders CN, Cota E, Baum J, Tate EW | 10.1021/acschembio.0c00328 |
| 32859024 | A De novo Peptide from a High Throughput Peptide Library Blocks Myosin A -MTIPComplex Formation in Plasmodium falciparum | Anam ZE, Joshi N, ..., Singh S | 10.3390/ijms21176158 |
| 28978649 | Reconstitution of the core of the malaria parasite glideosome with recombinantPlasmodium class XIV myosin A and Plasmodium actin | Bookwalter CS, Tay CL, ..., Trybus KM | 10.1074/jbc.M117.813972 |