Product Description: methyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.228 (tRNA (guanine(37)-N(1))-methyltransferase);2.5.1.114 (tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.09 | 0.96 |
| Trophozoite | expressed | 0.17 | 0.90 |
| Schizont | expressed | 0.05 | 0.97 |
| Gametocyte | expressed | 0.32 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1246500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -3.055 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1459600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4W7
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.59) with 2CWW (Global RMSD=2.04)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.- | Ex: homocysteine S-methyltransferase | EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate... |
| 2.1.1.228 | tRNA (guanine37-N1)-methyltransferase | BRENDA SOAP query unsuccessful |
| 2.5.1.114 | tRNAPhe (4-demethylwyosine37-C7) aminocarboxypropyltransferase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 29 | 19 | 36 |
| disruptive | 11 | 55 | 25 | 83 |
| missense | 9 | 50 | 25 | 77 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 62 | 32 | 40 |
| disruptive | 21 | 129 | 59 | 146 |
| missense | 11 | 110 | 54 | 115 |
PlasmoDB Total SNPs: 71
Non-coding: 26 | Synonymous: 27 | Nonsynonymous: 18 | Stop Codon: 0
Protein Length: 568 | Molecular Weight (kDa): 66.974
UniProt ID(s): Q8I4W7
PDB ID(s): None
Isoelectric Point: 8.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| InterPro | IPR030382 | SAM-dependent methyltransferase TRM5/TYW2-type |
| InterPro | IPR041532 | RlmI, PUA-like domain |
| PFam | PF02475 | SAM-dependent methyltransferase TRM5/TYW2-type |
| PFam | PF17785 | RlmI, PUA-like domain |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |