PF3D7_1247300

Genome location: Pf3D7_12_v3:1,951,571..1,955,002(+)

Genome classification: Core

Function and Localization

Product Description: GPI mannosyltransferase 2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 11

EC Numbers: 2.4.1.- (Hexosyltransferases.)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005789	endoplasmic reticulum membrane
Component	GO:0031501	mannosyltransferase complex
Function	GO:0000030	mannosyltransferase activity
Process	GO:0006506	GPI anchor biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.20	0.90
Trophozoite	expressed	0.22	0.85
Schizont	expressed	0.07	0.95
Gametocyte	expressed	0.16	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1247300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.045 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1460300)

Relative Growth Rate: 0.27 ± 0.23
Confidence: 4.34

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I4V9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.4.1.-	Ex: levansucrase	KIATPADPUDPAMPGTPGDPCTPUTPKClNEMIMP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103140

Most Similar Human Ortholog: A0A0A0MSX6

TM-align score: 0.87 | RMSD: 2.38

Seq Identity: 0.19 | Length: 178 / 531

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000060642	phosphatidylinositol glycan anchor biosynthesis class V

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	24	16	35
disruptive	9	54	34	84
missense	8	54	34	78

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	42	27	49
disruptive	16	132	57	115
missense	13	117	52	87

PlasmoDB Total SNPs: 75

Non-coding: 49 | Synonymous: 22 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 531 | Molecular Weight (kDa): 63.758

UniProt ID(s): Q8I4V9

PDB ID(s): None

Isoelectric Point: 8.87

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007315	GPI mannosyltransferase 2
PFam	PF04188	GPI mannosyltransferase 2
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097