PF3D7_1247500

Genome location: Pf3D7_12_v3:1,959,563..1,962,699(+)

Genome classification: Core

Function and Localization

Product Description: serine/threonine protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0030140	trans-Golgi network transport vesicle
Function	GO:0004693	cyclin-dependent protein serine/threonine kinase activity
Function	GO:0004674	protein serine/threonine kinase activity
Process	GO:0006468	protein phosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	not expressed	0.27	0.83
Schizont	expressed	0.81	0.55
Gametocyte	expressed	0.49	0.72

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1247500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.636 | #Insertions: 3

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1460500)

Relative Growth Rate: 0.87 ± 0.07
Confidence: 6.71

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1460500)

Modification: Disrupted | RMgm-4038

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I4V7

"Best" AlphaFill ligand hit: BU1 (1,4-butanediol, Local RMSD=0.15) with 7XZQ (Global RMSD=2.21)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O75716	Serine/threonine-protein kinase 16		OrthoDB	STK16-IN-1CHEMBL4062803CHEMBL4069365CHEMBL3719135...
Q9NSY1	BMP-2-inducible protein kinase	Homo sapiens	OrthoDB	CHEMBL516312CHEMBL4593649CHEMBL4531680CHEMBL4561238...
Q3UHJ0	AP2-associated protein kinase 1		OrthoDB	CHEMBL5193138CHEMBL5193012US9902722, Example 3US9902722, Example 2...
Q2M2I8	AP2-associated protein kinase 1	Homo sapiens	OrthoDB	US9708337, 1US9708337, 2US9708337, 3US9708337, 4...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101386

Most Similar Human Ortholog: K4DI97

TM-align score: 0.79 | RMSD: 2.88

Seq Identity: 0.30 | Length: 310 / 909

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000115977	AP2 associated kinase 1
ENSG00000138756	BMP2 inducible kinase

Protein Information

Protein Length: 909 | Molecular Weight (kDa): 105.537

UniProt ID(s): Q8I4V7

PDB ID(s): None

Isoelectric Point: 6.12

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
PFam	PF00069	Protein kinase domain
Superfamily	SSF56112	Protein kinase-like domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	44	24	61
disruptive	53	128	61	152
missense	34	107	59	135

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	86	25	61
disruptive	80	222	93	197
missense	52	187	79	144

PlasmoDB Total SNPs: 199

Non-coding: 40 | Synonymous: 96 | Nonsynonymous: 63 | Stop Codon: 0