PF3D7_1247600

Genome location: Pf3D7_12_v3:1,962,774..1,964,258(-)

Genome classification: Core

Function and Localization

Product Description: uroporphyrinogen-III synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 4.2.1.75 (Uroporphyrinogen-III synthase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.01	1.00
Trophozoite	no data	0.02	0.99
Schizont	no data	0.08	0.94
Gametocyte	no data	0.13	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1247600

Resistome Missense Mutations: F26S

Resistome Compounds with Missense Mutations: MMV019719

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.154 | MFS: -2.922 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1460600)

Relative Growth Rate: 1.04 ± 0.05
Confidence: 7.34

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1460600)

Modification: Disrupted | RMgm-4039

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A0S1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.2.1.75	uroporphyrinogen-III synthase	NH4+PbCl2iodoacetamidehydroxylaminePhenylglyoxalUroporphyrinsiodoacetic acidCoproporphyrinsN-ethylmaleimideButane-2,3-dionediethyl dicarbonatesulphydryl reagents...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532659

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	20	13	26
disruptive	8	43	26	69
missense	8	40	25	64

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	45	22	44
disruptive	21	104	59	123
missense	16	85	50	92

PlasmoDB Total SNPs: 31

Non-coding: 0 | Synonymous: 18 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 494 | Molecular Weight (kDa): 59.122

UniProt ID(s): A0A144A0S1

PDB ID(s): None

Isoelectric Point: 10.36

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily
InterPro	IPR039793	Uroporphyrinogen-III synthase
PFam	N/A	N/A
Superfamily	SSF69618	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily