PF3D7_1248000

Genome location: Pf3D7_12_v3:1,973,559..1,975,093(+)

Genome classification: Core

Function and Localization

Product Description: tRNA-splicing endonuclease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 4.6.1.16 (tRNA-intron lyase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000214	tRNA-intron endonuclease complex
Function	GO:0000213	tRNA-intron endonuclease activity
Process	GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation
Process	GO:0000379	tRNA-type intron splice site recognition and cleavage

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.98
Trophozoite	possibly expressed	0.24	0.84
Schizont	expressed	0.14	0.90
Gametocyte	possibly expressed	0.13	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1248000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.294 | MFS: -2.564 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A1E9

"Best" AlphaFill ligand hit: PH2 (2-amino-6-hydroxymethyl-7,8-dihydro-3h-pteridin-4-one, Local RMSD=0.20) with 6JWR (Global RMSD=14.62)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.6.1.16	tRNA-intron lyase	monovalent cationsp-hydroxymercuribenzoate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103858

Most Similar Human Ortholog: C9J7Z4

TM-align score: 0.44 | RMSD: 3.80

Seq Identity: 0.18 | Length: 123 / 220

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000154743	tRNA splicing endonuclease subunit 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	0	0	0
disruptive	0	0	0	0
missense	0	0	0	0

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	0	0	0
disruptive	0	0	0	0
missense	0	0	0	0

PlasmoDB Total SNPs: 55

Non-coding: 46 | Synonymous: 3 | Nonsynonymous: 5 | Stop Codon: 1

Protein Information

Protein Length: 220 | Molecular Weight (kDa): 26.639

UniProt ID(s): A0A144A1E9

PDB ID(s): None

Isoelectric Point: 4.95

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006676	tRNA-splicing endonuclease
InterPro	IPR006677	tRNA intron endonuclease, catalytic domain-like
InterPro	IPR036167	tRNA intron endonuclease, catalytic domain-like superfamily
PFam	PF01974	tRNA intron endonuclease, catalytic domain-like
Superfamily	SSF53032	tRNA intron endonuclease, catalytic domain-like superfamily