About Gene List

PF3D7_1248100

Genome location: Pf3D7_12_v3:1,975,145..1,976,734(+)

Genome classification: Core

Function and Localization

Product Description: methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.33 (tRNA (guanine(46)-N(7))-methyltransferase);2.1.1.43 (Transferred entry: 2.1.1.354)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.22 0.89
Trophozoite expressed 0.26 0.84
Schizont expressed 0.06 0.95
Gametocyte expressed 0.18 0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1248100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: 0.867 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1461000)

  • Relative Growth Rate: 1.00 ± 0.07
  • Confidence: 6.78

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1461000)

Modification: Disrupted | RMgm-4041

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I4V3

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.33) with 7EZG (Global RMSD=2.62)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.1.1.- Ex: homocysteine S-methyltransferase EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate...
2.1.1.33 tRNA (guanine46-N7)-methyltransferase MethylthioinosinemethylthioadenosineS-InosylhomocysteineS-adenosyl-L-cysteinep-hydroxymercuribenzoateSpleen phosphodiesteraseS-adenosyl-L-homocysteineS-Adenosyl-D-homocysteineS-adenosyl-DL-homocysteine
2.1.1.43 histone-lysine N-methyltransferase BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103169

Most Similar Human Ortholog: H7C552

TM-align score: 0.81 | RMSD: 1.81

Seq Identity: 0.46 | Length: 133 / 421

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000087995 methyltransferase like 2A
ENSG00000165055 methyltransferase like 2B

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 5 19 12 18
disruptive 21 64 41 71
missense 9 48 31 54

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 7 36 19 26
disruptive 35 126 69 116
missense 16 93 45 76

PlasmoDB Total SNPs: 68

Non-coding: 27 | Synonymous: 20 | Nonsynonymous: 21 | Stop Codon: 0

Protein Information

Protein Length: 421 | Molecular Weight (kDa): 49.946

UniProt ID(s): Q8I4V3

PDB ID(s): None

Isoelectric Point: 5.32

Protein Domain Annotations:

Source Family ID Description
InterPro IPR026113 Methyltransferase-like
InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase
PFam PF13489 N/A
Superfamily SSF53335 S-adenosyl-L-methionine-dependent methyltransferase

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108