PF3D7_1248200 (RBM22)

Genome location: Pf3D7_12_v3:1,977,477..1,978,969(+)

Genome classification: Core

Function and Localization

Product Description: pre-mRNA-splicing factor RBM22, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000974	Prp19 complex
Component	GO:0071006	U2-type catalytic step 1 spliceosome
Component	GO:0071007	U2-type catalytic step 2 spliceosome
Function	GO:0017070	U6 snRNA binding
Function	GO:0036002	pre-mRNA binding
Process	GO:0045292	mRNA cis splicing, via spliceosome

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.39	0.80
Trophozoite	expressed	0.32	0.80
Schizont	possibly expressed	0.16	0.89
Gametocyte	expressed	0.42	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1248200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.698 | MFS: -2.367 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1461100)

Relative Growth Rate: 0.23 ± 0.14
Confidence: 5.26

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I4V2

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102614

Most Similar Human Ortholog: E5RJW4

TM-align score: 0.79 | RMSD: 1.89

Seq Identity: 0.49 | Length: 156 / 357

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000086589	RNA binding motif protein 22

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	15	14	25
disruptive	9	30	29	57
missense	9	30	29	48

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	35	12	22
disruptive	12	90	33	88
missense	12	80	28	70

PlasmoDB Total SNPs: 47

Non-coding: 26 | Synonymous: 14 | Nonsynonymous: 5 | Stop Codon: 2

Protein Information

Protein Length: 357 | Molecular Weight (kDa): 41.968

UniProt ID(s): A0A144A0M5, Q8I4V2

PDB ID(s): None

Isoelectric Point: 9.03

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000504	RNA recognition motif domain
InterPro	IPR000571	Zinc finger, CCCH-type
InterPro	IPR035979	RNA-binding domain superfamily
InterPro	IPR036855	Zinc finger, CCCH-type superfamily
InterPro	IPR039171	Pre-mRNA-splicing factor Cwc2/Slt11
PFam	PF00076	RNA recognition motif domain
PFam	PF00642	Zinc finger, CCCH-type
Superfamily	SSF54928	RNA-binding domain superfamily
Superfamily	SSF90229	Zinc finger, CCCH-type superfamily