Genome location: Pf3D7_12_v3:2,002,325..2,004,499(-)
Genome classification: Core
Product Description: 26S protease regulatory subunit 8, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.8 (Transferred entry: 5.6.1.5)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0000502 | proteasome complex |
| Component | GO:0008540 | proteasome regulatory particle, base subcomplex |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003712 | transcription coregulator activity |
| Process | GO:0070682 | proteasome regulatory particle assembly |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.33 | 0.84 |
| Trophozoite | expressed | 1.08 | 0.45 |
| Schizont | expressed | 0.87 | 0.52 |
| Gametocyte | expressed | 0.54 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1248900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.988 | MFS: -2.052 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4U5
"Best" AlphaFill ligand hit: 08T ([[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium, Local RMSD=0.03) with 3U60 (Global RMSD=7.07)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.8 | proteasome ATPase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101513
Most Similar Human Ortholog: J3KRP2
TM-align score: 0.91 | RMSD: 2.03
Seq Identity: 0.75 | Length: 241 / 435
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000087191 | proteasome 26S subunit, ATPase 5 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 16 | 13 | 41 |
| disruptive | 1 | 10 | 6 | 22 |
| missense | 0 | 9 | 4 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 49 | 26 | 24 |
| disruptive | 2 | 24 | 18 | 58 |
| missense | 1 | 19 | 13 | 40 |
PlasmoDB Total SNPs: 73
Non-coding: 65 | Synonymous: 5 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 435 | Molecular Weight (kDa): 49.545
UniProt ID(s): Q8I4U5
PDB ID(s): None
Isoelectric Point: 7.01
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR003960 | ATPase, AAA-type, conserved site |
| InterPro | IPR005937 | 26S proteasome regulatory subunit P45-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR041569 | AAA ATPase, AAA+ lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF17862 | AAA ATPase, AAA+ lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |