Genome location: Pf3D7_12_v3:2,012,578..2,016,456(+)
Genome classification: Core
Product Description: protein phosphatase PPM4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0004724 | magnesium-dependent protein serine/threonine phosphatase activity |
| Function | GO:0004722 | protein serine/threonine phosphatase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.30 | 0.86 |
| Trophozoite | expressed | 0.73 | 0.59 |
| Schizont | expressed | 0.26 | 0.84 |
| Gametocyte | expressed | 0.63 | 0.65 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1249300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.547 | #Insertions: 5
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1462200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1462200)
Modification: Disrupted | RMgm-4045
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4U1
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=5.38) with 1MJH (Global RMSD=14.75)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 54 | 27 | 70 |
| disruptive | 59 | 188 | 87 | 205 |
| missense | 32 | 132 | 69 | 153 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 126 | 40 | 67 |
| disruptive | 111 | 387 | 167 | 403 |
| missense | 59 | 293 | 109 | 181 |
PlasmoDB Total SNPs: 237
Non-coding: 93 | Synonymous: 59 | Nonsynonymous: 85 | Stop Codon: 0
Protein Length: 1027 | Molecular Weight (kDa): 119.605
UniProt ID(s): Q8I4U1
PDB ID(s): None
Isoelectric Point: 4.38
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001932 | PPM-type phosphatase domain |
| InterPro | IPR015655 | Protein phosphatase 2C family |
| InterPro | IPR036457 | PPM-type phosphatase domain superfamily |
| PFam | PF00481 | PPM-type phosphatase domain |
| Superfamily | SSF81606 | PPM-type phosphatase domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 35083211 | Functional Characterization of 17 Protein Serine/Threonine Phosphatases inToxoplasma gondii Using CRISPR-Cas9 System | Liang QL, Nie LB, ..., Wang JL | 10.3389/fcell.2021.738794 |