Genome location: Pf3D7_12_v3:2,041,293..2,043,722(-)
Genome classification: Core
Product Description: ribosomal RNA small subunit methyltransferase A2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.182 (16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase);2.1.1.62 (mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0003677 | DNA binding |
| Function | GO:0000179 | rRNA (adenine-N6,N6-)-dimethyltransferase activity |
| Function | GO:0008649 | rRNA methyltransferase activity |
| Process | GO:0031167 | rRNA methylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.36 | 0.83 |
| Trophozoite | expressed | 0.84 | 0.55 |
| Schizont | expressed | 0.19 | 0.89 |
| Gametocyte | expressed | 0.46 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1249900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.59 | MFS: -2.445 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1462800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A0V8
"Best" AlphaFill ligand hit: MTA (5'-deoxy-5'-methylthioadenosine, Local RMSD=0.66) with 3FUW (Global RMSD=1.79)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.- | Ex: homocysteine S-methyltransferase | EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate... |
| 2.1.1.182 | 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase | protein S21 |
| 2.1.1.62 | mRNA (2'-O-methyladenosine-N6-)-methyltransferase | N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100569
Most Similar Human Ortholog: B4DRY2
TM-align score: 0.82 | RMSD: 1.81
Seq Identity: 0.29 | Length: 214 / 639
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000086189 | DIMT1 rRNA methyltransferase and ribosome maturation factor |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 26 | 19 | 41 |
| disruptive | 34 | 114 | 57 | 121 |
| missense | 14 | 78 | 44 | 74 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 55 | 32 | 32 |
| disruptive | 80 | 259 | 81 | 189 |
| missense | 38 | 152 | 56 | 110 |
PlasmoDB Total SNPs: 148
Non-coding: 26 | Synonymous: 69 | Nonsynonymous: 53 | Stop Codon: 0
Protein Length: 639 | Molecular Weight (kDa): 76.255
UniProt ID(s): A0A144A0V8
PDB ID(s): None
Isoelectric Point: 10.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001737 | Ribosomal RNA adenine methyltransferase KsgA/Erm |
| InterPro | IPR011530 | Ribosomal RNA adenine dimethylase |
| InterPro | IPR020596 | Ribosomal RNA adenine methylase transferase, conserved site |
| InterPro | IPR020598 | Ribosomal RNA adenine methylase transferase, N-terminal |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF00398 | Ribosomal RNA adenine methyltransferase KsgA/Erm |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |