Genome location: Pf3D7_12_v3:2,045,070..2,054,429(-)
Genome classification: Core
Product Description: osmiophilic body protein G377
SignalP Peptide: MKLLLFFFISSIFLQLTSG
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005634 | nucleus |
| Component | GO:0044310 | osmiophilic body |
| Process | GO:0035891 | exit from host cell |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.07 | 0.97 |
| Trophozoite | possibly expressed | 0.04 | 0.98 |
| Schizont | possibly expressed | 0.08 | 0.96 |
| Gametocyte | expressed | 0.55 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1250100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.976 | #Insertions: 6
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1463000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1463000)
Modification: Disrupted | RMgm-4047
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 142 | 84 | 193 |
| disruptive | 86 | 294 | 185 | 454 |
| missense | 60 | 252 | 172 | 393 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 49 | 269 | 142 | 213 |
| disruptive | 140 | 596 | 299 | 597 |
| missense | 97 | 496 | 268 | 497 |
PlasmoDB Total SNPs: 270
Non-coding: 0 | Synonymous: 145 | Nonsynonymous: 125 | Stop Codon: 0
Protein Length: 3119 | Molecular Weight (kDa): 377.386
UniProt ID(s): A0A059WCL7, A0A059WCM1, A0A059WHL4, A0A059WHL9, A0A059WIJ2, A0A059WIJ5, A0A059WIK1, A0A059WNE6, Q25857, Q26022, Q26023, Q8I4T3
PDB ID(s): None
Isoelectric Point: 6.07
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 8719156 | COS cell expression cloning of Pfg377, a Plasmodium falciparum gametocyte antigenassociated with osmiophilic bodies. | Alano P, Read D, ..., Elliott JF | 10.1016/0166-6851(95)02491-3 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18086189 | The role of osmiophilic bodies and Pfg377 expression in female gametocyteemergence and mosquito infectivity in the human malaria parasite Plasmodiumfalciparum. | de Koning-Ward TF, Olivieri A, ..., Ranford-Cartwright LC | 10.1111/j.1365-2958.2007.06039.x |
| 25262869 | Distinct properties of the egress-related osmiophilic bodies in male and femalegametocytes of the rodent malaria parasite Plasmodium berghei. | Olivieri A, Bertuccini L, ..., Ponzi M | 10.1111/cmi.12370 |
| 27432909 | Comparative Proteomics and Functional Analysis Reveal a Role of Plasmodiumfalciparum Osmiophilic Bodies in Malaria Parasite Transmission. | Suarez-Cortes P, Sharma V, ..., Alano P | 10.1074/mcp.M116.060681 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 27469366 | Autophagy confers resistance to lipopolysaccharide-induced mouse hepatocyteinjury | Lalazar G, Ilyas G, ..., Czaja MJ | 10.1152/ajpgi.00124.2016 |
| 34869075 | Characterization of Naturally Acquired Immunity to a Panel of Antigens Expressedin Mature P. falciparum Gametocytes | Muthui MK, Takashima E, ..., Kapulu MC | 10.3389/fcimb.2021.774537 |
| 27427910 | A Putative Small Solute Transporter Is Responsible for the Secretion of G377 andTRAP-Containing Secretory Vesicles during Plasmodium Gamete Egress and SporozoiteMotility | Kehrer J, Singer M, ..., Mair GR | 10.1371/journal.ppat.1005734 |
| 31981605 | Observation of morphological changes of female osmiophilic bodies prior toPlasmodium gametocyte egress from erythrocytes | Ishino T, Tachibana M, ..., Torii M | 10.1016/j.molbiopara.2020.111261 |
| 37492994 | Comparative proteomics of vesicles essential for the egress of Plasmodiumfalciparum gametocytes from red blood cells | Sassmannshausen J, Bennink S, ..., Pradel G | 10.1111/mmi.15125 |