PF3D7_1250800

Genome location: Pf3D7_12_v3:2,077,623..2,083,307(+)

Genome classification: Core

Function and Localization

Product Description: DNA repair protein rhp16, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000109	nucleotide-excision repair complex
Function	GO:0005524	ATP binding
Function	GO:0004386	helicase activity
Process	GO:0006289	nucleotide-excision repair

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.29	0.86
Trophozoite	expressed	0.94	0.51
Schizont	expressed	0.30	0.83
Gametocyte	possibly expressed	0.53	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1250800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.803 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1463700)

Relative Growth Rate: 0.87 ± 0.26
Confidence: 4.10

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1463700)

Modification: Disrupted | RMgm-4052

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I4S6

"Best" AlphaFill ligand hit: KH2 (3-(1-methylpiperidinium-1-yl)propane-1-sulfonate, Local RMSD=1.30) with 5HZR (Global RMSD=2.85)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105241

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	25	98	58	98
disruptive	51	205	126	304
missense	39	182	111	254

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	38	206	68	110
disruptive	93	429	188	377
missense	65	362	158	283

PlasmoDB Total SNPs: 253

Non-coding: 122 | Synonymous: 66 | Nonsynonymous: 65 | Stop Codon: 0

Protein Information

Protein Length: 1647 | Molecular Weight (kDa): 192.172

UniProt ID(s): Q8I4S6

PDB ID(s): None

Isoelectric Point: 8.86

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000330	SNF2-related, N-terminal domain
InterPro	IPR001510	Zinc finger, PARP-type
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR001841	Zinc finger, RING-type
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00176	SNF2-related, N-terminal domain
PFam	PF00271	Helicase, C-terminal
PFam	PF13639	Zinc finger, RING-type
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase
Superfamily	SSF57716
Superfamily	SSF57850

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104