Genome location: Pf3D7_12_v3:2,099,430..2,102,248(+)
Genome classification: Core
Product Description: ATP-dependent RNA helicase DRS1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005730 | nucleolus |
| Component | GO:0030687 | preribosome, large subunit precursor |
| Process | GO:0000027 | ribosomal large subunit assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.70 | 0.67 |
| Trophozoite | expressed | 0.42 | 0.77 |
| Schizont | not expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.32 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1251500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.616 | MFS: -1.164 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1464500)
Modification: Other | RMgm-437
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A382
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.02) with 5JCH (Global RMSD=2.57)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q2FUW6 | Protein translocase subunit SecA 2 | OrthoMCL BLAST | Rose bengal sodium |
Ortholog Group (OrthoMCL): OG6_102067
Most Similar Human Ortholog: B7Z6D5
TM-align score: 0.59 | RMSD: 2.37
Seq Identity: 0.36 | Length: 439 / 705
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000124228 | DEAD-box helicase 27 |
Protein Length: 705 | Molecular Weight (kDa): 82.582
UniProt ID(s): A0A144A382, A7U5X2
PDB ID(s): None
Isoelectric Point: 10.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000629 | ATP-dependent RNA helicase DEAD-box, conserved site |
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 50 | 24 | 41 |
| disruptive | 16 | 118 | 54 | 109 |
| missense | 12 | 112 | 54 | 101 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 85 | 31 | 56 |
| disruptive | 40 | 211 | 79 | 167 |
| missense | 30 | 182 | 75 | 123 |
PlasmoDB Total SNPs: 106
Non-coding: 54 | Synonymous: 26 | Nonsynonymous: 26 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 11911362 | Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of60S ribosomal subunits | Adams CC, Jakovljevic J, ..., Woolford JL Jr | 10.1017/s1355838202010026 |
| 16449635 | Comprehensive mutational analysis of yeast DEXD/H box RNA helicases involved inlarge ribosomal subunit biogenesis | Bernstein KA, Granneman S, ..., Baserga SJ | 10.1128/MCB.26.4.1195-1208.2006 |